Label-free Electrochemical Immunosensor for Sensitive Detection of Rheumatoid Arthritis Biomarker Anti-CCP-ab

被引：8
|
作者：
Ma, Jing [1 ]
Li, Dai [1 ]
Sun, Bolu [1 ]
Hou, Xiaohui [1 ]
Zhang-Peng, Xinru [1 ]
Li, Wen [1 ]
Zhang, Yan [3 ]
Hu, Fangdi [1 ]
Shi, Xiaofeng [2 ]
机构：
[1] Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China
[2] Gansu Acad Med Sci, Xiaoxihu East St, Lanzhou 730050, Gansu, Peoples R China
[3] Dong E E iao Co Ltd, Natl Engn Res Ctr Gelatin Based Tradit Chinese Me, Liaocheng, Shandong, Peoples R China
来源：
ELECTROANALYSIS | 2022年 / 34卷 / 04期
基金：
中国国家自然科学基金; 中央高校基本科研业务费专项资金资助;
关键词：
Label-free immunosensor; Rheumatoid arthritis biomarkers; Anti-cyclic citrullinated peptide antibody; Nitrogen-doped graphene; Gold nanoparticles; CYCLIC CITRULLINATED PEPTIDE; NITROGEN-DOPED GRAPHENE; POSTTRANSLATIONAL MODIFICATION; DNA; DIAGNOSIS; IMMUNOASSAY; PERFORMANCE; ANTIBODIES; NANOSHEETS; GROWTH;
D O I：
10.1002/elan.202100045
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The accurate and sensitive analysis of rheumatoid arthritis(RA) trace biomarkers is essential for early warning and diagnosis of RA, while anti-cyclic citrullinated peptide antibody (anti-CCP-ab) is a specific one. In this study, a simple label-free electrochemical immunosensor for the detection of anti-CCP-ab was constructed with nitrogen-doped graphene (N-G) and gold nanoparticles (AuNPs). The proposed non-enzyme immunosensor had exhibited high specificity, selectivity, and stability in the linear calibration curve range from 0.125 similar to 2000 pg mL-1 and has been successfully used in the detection of anti-CCP-ab in human serum, providing a new idea for the early diagnosis of RA.
引用
收藏
页码：761 / 771
页数：11
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