Differences in Vitreous Protein Profiles in Patients With Proliferative Diabetic Retinopathy Before and After Ranibizumab Treatment

被引:3
作者
She, Xinping [1 ]
Zou, Chen [2 ]
Zheng, Zhi [1 ]
机构
[1] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Natl Clin Res Ctr Eye Dis, Shanghai Engn Ctr Visual Sci & Photomed,Sch Med,Sh, Shanghai, Peoples R China
[2] Fudan Univ, Eye & ENT Hosp, Eye Inst, Shanghai Med Coll, Shanghai, Peoples R China
基金
中国国家自然科学基金;
关键词
proliferative diabetic retinopathy; proteomics; ranibizumab; label-free; LC-MS; MS; PRM; BINDING; IDENTIFICATION; THERAPY; GENES; RISK;
D O I
10.3389/fmed.2022.776855
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Proliferative diabetic retinopathy (PDR) accounts for severe impact on vision, its mechanism is still poorly understood. To compare the differences of vitreous protein profiles in PDR patients before and after a complete anti-vascular endothelial growth factor (VEGF) loading dose with ranibizumab treatment. Twelve vitreous humor (VH) samples were collected from six PDR patients before (set as pre group) and after (set as post group) intravitreal injection of ranibizumab (IVR) treatment. LC-MS/MS and bioinformatics analysis were performed to identify differentially expressed proteins. Proteins were validated with targeted proteomics using parallel reaction monitoring (PRM) in a validation set consisting of samples from the above patients. A total of 2680 vitreous proteins were identified. Differentially expressed proteins were filtrated with fold change >= 2.0 (post group/ pre group protein abundance ratio >= 2 or <= 0.5) and p-value <0.05. 11 proteins were up-regulated and 17 proteins were down-regulated, while consistent presence/absence expression profile group contains one elevated protein and nine reduced proteins, among which seven proteins were identified as potential biomarkers for IVR treatment through PRM assays. Bioinformatics analysis indicated the up-regulated proteins were significantly enriched in "GnRH secretion" and "Circadian rhythm" signaling pathway. This report represents the first description of combined label-free quantitative proteomics and PRM analysis of targeted proteins for discovery of different proteins before and after IVR treatment in the same patient. IVR treatment may protect against PDR by promoting SPP1 expression through "GnRH secretion" and "Circadian rhythm" signaling pathway.
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页数:12
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