COMPARISON STUDY OF INTEGRIN B2 GENE EXPRESSIONS BETWEEN ACUTE MYOCARDIAL INFARCTION AND STABLE ANGINA PECTORIS PATIENTS

Introduction: To systemically compare the characteristics of integrin beta 2 signaling pathways related gene expressions among acute myocardial infarction (AMI) patients, stable angina pectoris (SA) patients and healthy controls. Materials and methods: 20 AMI patients, SA patients and healthy people were enrolled into AMI, SA and control groups respectively. Gene expressions associated with signaling pathways of integrin 132 of three groups were detected and compared by t test. Results: Compared with controls, mRNA expressions of alpha/beta subunit (ITGAD, ITGAL, ITGAM, ITGAX and ITGB2), ligands of integrin beta 2 (RAGE, JAM-1, Fibrinogen, ICAM-1, ICAM-3, ICAM-5 and uPAR), chemokine (fMLP and RAF), inside-out signaling pathway (RIAM, ADAP, SLP-76, PLC gamma, RAPL, SPA1, alpha-actinin, calpain, Dok1, radixin, talin and 14-3-3 zeta) and outside-in signaling pathway (Fgr, Hck, Lyn, PKC, c-abl, Syk, Vav1/3, cdc42, mDia, Rac, RhoA and ROCK1/2) in AMI patients were significantly upregulated. However there was no significant difference of gene expressions of integrin beta 2 and its signaling pathway in SA patients compared to control group. Conclusions: The inside-out signaling pathway was downregulated generally in AMI patients, but not in SA patients. However, the outside-in signaling pathway was activated to be upregulated in AMI patients, but not in SA patients. Thus, the activation of integrin beta 2 was irritated by external factors.