Advances of endomicroscopy for gastrointestinal physiology and diseases

被引:48
作者
Goetz, Martin [1 ]
Kiesslich, Ralf [1 ]
机构
[1] Univ Med Mainz, Med Klin & Poliklin 1, D-55131 Mainz, Germany
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2010年 / 298卷 / 06期
关键词
molecular imaging; Barrett's esophagus; gastric cancer; celiac disease; ulcerative colitis; colorectal cancer; CONFOCAL LASER ENDOMICROSCOPY; IN-VIVO DIAGNOSIS; INTRAEPITHELIAL NEOPLASIA; FLUORESCENCE MICROSCOPY; COLLAGENOUS COLITIS; COLORECTAL-CANCER; ANIMAL-MODELS; MINIPROBE; HISTOLOGY; LIVER;
D O I
10.1152/ajpgi.00027.2010
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Goetz M, Kiesslich R. Advances of endomicroscopy for gastrointestinal physiology and diseases. Am J Physiol Gastrointest Liver Physiol 298: G797-G806, 2010. First published February 25, 2010; doi:10.1152/ajpgi.00027.2010.-Confocal endomicroscopy is a novel technique that permits in vivo microscopy of the human gastrointestinal mucosa during ongoing endoscopy, thereby providing optical virtual biopsies. Endomicroscopy has been demonstrated to reveal histological information in a multitude of diseases in the upper and lower gastrointestinal tract in vivo. Most studies have focused on inflammation and neoplasia, such as Barrett's esophagus, gastric cancer, celiac disease, Crohn's disease and ulcerative colitis, or colorectal neoplasias. Endomicroscopy allows obtainment of "smart," targeted biopsies from regions with microscopic alterations rather than having to rely on random untargeted tissue sampling. This reduces the number of biopsies while increasing the diagnostic yield. In addition, immediate histological information is available, enabling immediate therapy. Apart from morphological visualization, endomicroscopy offers a unique possibility to study pathophysiological events in their natural environment (functional imaging). Molecular imaging with endomicroscopy applied in clinical and basic science will permit advances in understanding of the cellular basis of gastrointestinal physiology and pathophysiology.
引用
收藏
页码:G797 / G806
页数:10
相关论文
共 52 条
[1]   In vivo detection of small subsurface melanomas in athymic mice using noninvasive fiber optic confocal imaging [J].
Anikijenko, P ;
Vo, LT ;
Murr, ER ;
Carrasco, J ;
McLaren, WJ ;
Chen, QY ;
Thomas, SG ;
Delaney, PM ;
King, RG .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 117 (06) :1442-1448
[2]   Confocal laser scanning fluorescence microscopy for in vivo determination of microvessel density in Barrett's esophagus [J].
Becker, V. ;
Vieth, M. ;
Bajbouj, M. ;
Schmid, R. M. ;
Meining, A. .
ENDOSCOPY, 2008, 40 (11) :888-891
[3]   In vivo diagnosis of acute intestinal graft-versus-host disease by confocal endomicroscopy [J].
Bojarski, C. ;
Guenther, U. ;
Rieger, K. ;
Heller, F. ;
Loddenkemper, C. ;
Gruenbaum, M. ;
Uharek, L. ;
Zeitz, M. ;
Hoffmann, J. C. .
ENDOSCOPY, 2009, 41 (05) :433-438
[4]   In-vivo microvascular imaging of early squamous-cell cancer of the esophagus by conflocal laser endomicroscopy [J].
Deinert, K. ;
Kiesslich, R. ;
Vieth, M. ;
Neurath, M. F. ;
Neuhaus, H. .
ENDOSCOPY, 2007, 39 (04) :366-368
[5]  
Dunbar KB, 2008, GASTROENTEROLOGY, V134, pA62
[6]   Confocal laser endomicroscopy in Barrett's esophagus and endoscopically inapparent Barrett's neoplasia: a prospective, randomized, double-blind, controlled, crossover trial [J].
Dunbar, Kerry B. ;
Okolo, Patrick, III ;
Montgomery, Elizabeth ;
Canto, Marcia Irene .
GASTROINTESTINAL ENDOSCOPY, 2009, 70 (04) :645-654
[7]  
FOTTNER C, ENDOCRINOLO IN PRESS
[8]   Dynamic in vivo Imaging of Microvasculature and Perfusion by Miniaturized Confocal Laser Microscopy [J].
Goetz, M. ;
Thomas, S. ;
Heimann, A. ;
Delaney, P. ;
Schneider, C. ;
Relle, M. ;
Schwarting, A. ;
Galle, P. R. ;
Kempski, O. ;
Neurath, M. F. ;
Kiesslich, R. .
EUROPEAN SURGICAL RESEARCH, 2008, 41 (03) :290-297
[9]   In vivo confocal laser endomicroscopy of the human liver: a novel method for assessing liver microarchitecture in real time [J].
Goetz, M. ;
Kiesslich, R. ;
Dienes, H. -P. ;
Drebber, U. ;
Murr, E. ;
Hoffman, A. ;
Kanzler, S. ;
Galle, P. R. ;
Delaney, P. ;
Neurath, M. F. .
ENDOSCOPY, 2008, 40 (07) :554-562
[10]   In-vivo confocal real-time mini-microscopy in animal models of human inflammatory and neoplastic diseases [J].
Goetz, M. ;
Fottner, C. ;
Schirrmacher, E. ;
Delaney, P. ;
Gregor, S. ;
Schneider, C. ;
Strand, D. ;
Kanzler, S. ;
Memadathil, B. ;
Weyand, E. ;
Holtmann, M. ;
Schirrmacher, R. ;
Weber, M. M. ;
Anlauf, M. ;
Kloeppel, G. ;
Vieth, M. ;
Galle, P. R. ;
Bartenstein, P. ;
Neurath, M. F. ;
Kiesslich, R. .
ENDOSCOPY, 2007, 39 (04) :350-356