Alcaligenes eutrophus cells containing hydrogenase, a coenzyme regenerating catalyst for NADH-dependent oxidoreductases

被引：6

作者：

Andersson, M

Otto, R

Holmberg, H

Adlercreutz, P

机构：

[1] Univ Lund, Ctr Chem & Chem Engn, Dept Biotechnol, S-22100 Lund, Sweden

[2] Univ Stuttgart, Dept Biochem Engn, D-7000 Stuttgart, Germany

[3] Synthelec AB, Lund, Sweden

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 1997年 / 15卷 / 04期

关键词：

Alcaligenes eutrophus; hydrogenase; NADH-regeneration; HLADH; LDH;

D O I：

10.3109/10242429709003195

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alcaligenes eutrophus cells containing a soluble NAD-dependent hydrogenase was evaluated as a NADH regenerating catalyst. In the presence of hydrogen and permeabilized A. eutrophus cells, NADH regeneration was achieved, and reductions catalyzed by horse liver alcohol dehydrogenase and lactate dehydrogenase proceeded to 90-99% conversion. The operational stability of the hydrogenase was improved by the addition of reducing agents such as dithiothreitol and by immobilization of the cells in calcium alginate.

引用

页码：281 / 296

页数：16

共 15 条

[1] Cofactor regeneration in biocatalysis in organic media [J].