Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2

被引:272
作者
Iohara, K
Nakashima, M
Ito, M
Ishikawa, M
Nakasima, A
Akamine, A
机构
[1] Kyushu Univ, Fac Dent Sci, Dept Clin Oral Mol Biol, Div Oral Rehabil,Higashi Ku, Fukuoka 8128582, Japan
[2] Kyushu Univ, Fac Dent Sci, Dept Orthodont, Div Oral Hlth Growth & Dev,Higashi Ku, Fukuoka 8128582, Japan
[3] Natl Def Med Coll, Dept Anat 1, Tokorozawa, Saitama 3598513, Japan
关键词
dentin regeneration; stem cell therapy; BMP2; dental pulp-capping; pellet culture;
D O I
10.1177/154405910408300802
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin regeneration. Culture of porcine pulp cells, as a three-dimensional pellet, promoted odontoblast differentiation compared with monolayers. The expression of dentin sialophosphoprotein ( Dspp) and enamelysin/matrix metalloproteinase 20 (MMP20) mRNA confirmed the differentiation of pulp cells into odontoblasts and was stimulated by the morphogenetic signal, bone morphogenetic protein 2 (BMP2). Based on the in vitro experiments, an in vivo evaluation of pulp progenitor/stem cells in the dog was performed. The autogenous transplantation of the BMP2-treated pellet culture onto the amputated pulp stimulated reparative dentin formation. In conclusion, BMP2 can direct pulp progenitor/stem cell differentiation into odontoblasts and result in dentin formation.
引用
收藏
页码:590 / 595
页数:6
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