Sample Preparation for Plant Metabolomics

被引：233

作者：

Kim, Hye Kyong ^{[1
]}

Verpoorte, Rob ^{[1
]}

机构：

[1] Leiden Univ, Inst Biol, Div Pharmacognosy, Sect Metabol, NL-2333 CC Leiden, Netherlands

来源：

PHYTOCHEMICAL ANALYSIS | 2010年 / 21卷 / 01期

关键词：

Plant metabolomics; sample preparation procedure; harvesting; drying; extraction; extraction solvent; analytical methods; MAGNETIC-RESONANCE-SPECTROSCOPY; MULTIVARIATE STATISTICAL-ANALYSIS; MICROWAVE-ASSISTED EXTRACTION; PRINCIPAL COMPONENT ANALYSIS; MASS-SPECTROMETRY; LASER MICRODISSECTION; H-1-NMR SPECTROSCOPY; MEDICINAL-PLANTS; NMR-SPECTROSCOPY; WATER ACTIVITY;

D O I：

10.1002/pca.1188

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sample preparation in plant metabolomics is a fundamental and critical step with important consequences for the accuracy of results. Depending on the analytical tools and the metabolites of interest, sample preparation has to be decided. However, the various methods reported in the literature have many steps in common and consequently the practical considerations concerning the pros and cons are similar. In this review, each step of the sample preparation - harvesting, drying, extraction and purification - will be discussed in detail. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：4 / 13

页数：10

共 55 条

[1] Metabolic characterization of Brassica rapa leaves by NMR spectroscopy [J].

Abdel-Farid, Brahim Bayoumi ;

Kim, Hye Kyong ;

Choi, Young Hae ;

Verpoorte, Robert .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (19) :7936-7943

[2] Use of high-resolution magic angle spinning nuclear magnetic resonance spectroscopy for in situ studies of low-molecular-mass compounds in red algae [J].

Broberg, A ;

Kenne, L .

ANALYTICAL BIOCHEMISTRY, 2000, 284 (02) :367-374

[3] Improved water elimination in NOESY experiments by radiofrequency field gradients [J].

Brondeau, J ;

Mutzenhardt, P ;

Tyburn, JM ;

Canet, D .

JOURNAL OF MAGNETIC RESONANCE SERIES B, 1995, 109 (03) :310-313

[4] Metabolic fingerprinting of wild type and transgenic tobacco plants by 1H NMR and multivariate analysis technique [J].

Choi, HK ;

Choi, YH ;

Verberne, M ;

Lefeber, AWM ;

Erkelens, C ;

Verpoorte, R .

PHYTOCHEMISTRY, 2004, 65 (07) :857-864

[5] Metabolic discrimination of Catharanthus roseus leaves infected by phytoplasma using 1H-NMR spectroscopy and multivariate data analysis [J].

Choi, YH ;

Tapias, EC ;

Kim, HK ;

Lefeber, AWM ;

Erkelens, C ;

Verhoeven, JTJ ;

Brzin, J ;

Zel, J ;

Verpoorte, R .

PLANT PHYSIOLOGY, 2004, 135 (04) :2398-2410

[6] Metabolomic differentiation of Cannabis sativa cultivars using 1H NMR spectroscopy and principal component analysis [J].

Choi, YH ;

Kim, HK ;

Hazekamp, A ;

Erkelens, C ;

Lefeber, AWM ;

Verpoorte, R .

JOURNAL OF NATURAL PRODUCTS, 2004, 67 (06) :953-957

[7] Classification of commercial catuaba samples by NMR, HPLC and chemometrics [J].

Daolio, Cristina ;

Beltrame, Flavio L. ;

Ferreira, Antonio G. ;

Cass, Quezia B. ;

Garcia Cortez, Diogenes Aparicio ;

Ferreira, Marcia M. C. .

PHYTOCHEMICAL ANALYSIS, 2008, 19 (03) :218-228

[8] Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry [J].

De Vos, Ric C. H. ;

Moco, Sofia ;

Lommen, Arjen ;

Keurentjes, Joost J. B. ;

Bino, Raoul J. ;

Hall, Robert D. .

NATURE PROTOCOLS, 2007, 2 (04) :778-791

[9] Current trends and future requirements for the mass spectrometric investigation of microbial, mammalian and plant metabolomes [J].

Dunn, Warwick B. .

PHYSICAL BIOLOGY, 2008, 5 (01)

[10] Pre-analytical method for metabolic profiling of plant cell cultures of Passiflora garckei [J].

Fraccaroli, Marica ;

Nicoletti, Silvia ;

Maltese, Federica ;

Choi, Young Hae ;

Guzzo, Flavia ;

Levi, Marisa ;

Verpoorte, Robert .

BIOTECHNOLOGY LETTERS, 2008, 30 (11) :2031-2036

← 1 2 3 4 5 6 →