A novel label-free electrochemical immunosensor based on graphene and thionine nanocomposite

被引：109

作者：

Wei, Qin ^{[1
]}

Mao, Kexia ^{[1
]}

Wu, Dan ^{[1
]}

Dai, Yuxue ^{[1
]}

Yang, Jian ^{[1
]}

Du, Bin ^{[1
]}

Yang, Minghui ^{[1
]}

Li, He ^{[1
]}

机构：

[1] Univ Jinan, Sch Chem & Chem Engn, Jinan 250022, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2010年 / 149卷 / 01期

关键词：

Alpha fetoprotein; Cancer marker; Label-free; Graphene; Thionine; ALPHA-FETOPROTEIN; IMMUNOASSAY; NANOSHEETS; CELLS; SERUM;

D O I：

10.1016/j.snb.2010.06.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel label-free electrochemical immunosensor for the detection of alpha fetoprotein (AFP) has been developed by immobilizing anti-AFP on graphene sheet (GS) and thionine (TH) modified glassy carbon electrode. The modification procedure was characterized by cyclic voltammetry (CV). The detection is based on the peak current change of the modified electrode before and after the antigen-antibody reaction. Tests relating to the detection of AFP demonstrated that the immunosensor exhibited high sensitivity, wide linear range (0.05-2.00 ng mL(-1)), low detection limit (5.77 pg mL(-1) based S/N = 3) and good long-term stability. The reliability of the developed immunosensor has been proved by the detection of AFP in serum samples with satisfactory results. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：314 / 318

页数：5

共 25 条

[1] Metal decorated graphene nanosheets as immobilization matrix for amperometric glucose biosensor [J].

Baby, Tessy Theres ;

Aravind, S. S. Jyothirmayee ;

Arockiadoss, T. ;

Rakhi, R. B. ;

Ramaprabhu, S. .

SENSORS AND ACTUATORS B-CHEMICAL, 2010, 145 (01) :71-77

[2] An electrochemical impedance immunosensor with signal amplification based on Au-colloid labeled antibody complex [J].

Chen, Huan ;

Jiang, Jian-Hui ;

Huang, Yong ;

Deng, Ting ;

Li, Ji-Shan ;

Shen, Guo-Li ;

Yu, Ru-Qin .

SENSORS AND ACTUATORS B-CHEMICAL, 2006, 117 (01) :211-218

[3] Rapid analysis of alpha-fetoprotein by chemiluminescence microfluidic immunoassay system based on super-paramagnetic microbeads [J].

Huang, Hui ;

Zheng, Xiao Lin ;

Zheng, Jun Song ;

Pan, Jing ;

Pu, Xiao Yun .

BIOMEDICAL MICRODEVICES, 2009, 11 (01) :213-216

[4] Nano-ELISA for highly sensitive protein detection [J].

Jia, Chun-Ping ;

Zhong, Xiao-Qin ;

Hua, Bao ;

Liu, Mei-Ying ;

Jing, Feng-Xiang ;

Lou, Xin-Hui ;

Yao, Shi-Hua ;

Xiang, Jia-Qing ;

Jin, Qing-Hui ;

Zhao, Jian-Long .

BIOSENSORS & BIOELECTRONICS, 2009, 24 (09) :2836-2841

[5] Electrochemiluminescence immunosensor based on CdSe nanocomposites [J].

Jie, Guifen ;

Zhang, Jingjing ;

Wang, Danchen ;

Cheng, Chao ;

Chen, Hong-Yuan ;

Zhu, Jun-Jie .

ANALYTICAL CHEMISTRY, 2008, 80 (11) :4033-4039

[6] Amperometric biosensors based on redox polymer-carbon nanotube-enzyme composites [J].

Joshi, PP ;

Merchant, SA ;

Wang, YD ;

Schmidtke, DW .

ANALYTICAL CHEMISTRY, 2005, 77 (10) :3183-3188

[7] Posaconazole [J].

Kauffman, Carol A. ;

Malani, Anuray N. ;

Easley, Chris ;

Kirkpatrick, Peter .

NATURE REVIEWS DRUG DISCOVERY, 2007, 6 (03) :183-184

[8] Label-free electrochemical immunoassay for the detection of human chorionic gonadotropin hormone [J].

Kerman, Kagan ;

Nagatani, Naoki ;

Chikae, Miyuki ;

Yuhi, Teruko ;

Takamura, Yuzuru ;

Tamiya, Eiichi .

ANALYTICAL CHEMISTRY, 2006, 78 (15) :5612-5616

[9] Graphene modified basal and edge plane pyrolytic graphite electrodes for electrocatalytic oxidation of hydrogen peroxide and β-nicotinamide adenine dinucleotide [J].

Lin, Wei-Jhih ;

Liao, Chien-Shiun ;

Jhang, Jia-Hao ;

Tsai, Yu-Chen .

ELECTROCHEMISTRY COMMUNICATIONS, 2009, 11 (11) :2153-2156

[10] Membraneless enzymatic biofuel cells based on graphene nanosheets [J].

Liu, Chang ;

Alwarappan, Subbiah ;

Chen, Zhongfang ;

Kong, Xiangxing ;

Li, Chen-Zhong .

BIOSENSORS & BIOELECTRONICS, 2010, 25 (07) :1829-1833

← 1 2 3 →