Retrotransposon-based sequence-specific amplified polymorphism markers for the analysis of genetic diversity and phylogeny in Malus Mill. (Rosaceae)

被引：4
作者：
Savelyeva, Ekaterina [1 ]
Kalegina, Anna [1 ]
Boris, Ksenia [1 ,2 ]
Kochieva, Elena [2 ]
Kudryavtsev, Alexander [1 ]
机构：
[1] Russian Acad Sci, Vavilov Inst Gen Genet, Gubkin St 3, Moscow 119991, Russia
[2] Russian Acad Sci, Inst Bioengn, Res Ctr Biotechnol, Leninsky Ave,33,Bld 2, Moscow 119071, Russia
来源：
GENETIC RESOURCES AND CROP EVOLUTION | 2017年 / 64卷 / 07期
基金：
俄罗斯科学基金会;
关键词：
Apple; Malus genus; S-SAP-markers; LTR-retrotransposon; Genetic diversity; Phylogeny; S-SAP MARKERS; DOMESTICATED APPLE; GENUS MALUS; MOLECULAR MARKERS; EVOLUTIONARY; SYLVESTRIS; RESISTANCE; SSAP; HISTORY; LINKAGE;
D O I：
10.1007/s10722-016-0449-1
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Sequence-Specific Amplified Polymorphism (S-SAP)-analysis of 131 accessions of different species from five sections of the genus Malus was carried out to study genetic diversity, and to clarify phylogenetic and taxonomic issues. S-SAP-markers, based on long terminal repeat (LTR)-retrotransposons TRIM2 and dem1, were developed, which identified 679 polymorphic fragments in the studied Malus species. S-SAP technique proved to be effective for taxonomic studies in Malus. The obtained results generally support the existing sectional taxonomy in the genus Malus and allowed to determine the taxonomic status of some Russian landraces. The genetic diversity and taxonomic status of the Russian apple Antonovka landraces, widely used in breeding programs for their increased adaptation to abiotic stress and scab resistance, were clarified. All of them belong to M. domestica, section Malus. However, Antonovka Olginskaya might have a hybrid origin with some contribution from Gymnomeles species according to PCO analysis data. The taxonomic status was resolved for another Russian landrace, Yakutskaya, which exhibits increased winter hardiness and drought resistance; it belongs to section Gymnomeles, with a high resemblance to M. baccata.
引用
收藏
页码：1499 / 1511
页数：13
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