Human Spheroids from Adipose-Derived Stem Cells Induce Calvarial Bone Production in a Xenogeneic Rabbit Model

被引：11

作者：

Di Stefano, Anna Barbara ^{[1
]}

Montesano, Luigi ^{[1
,2
]}

Belmonte, Beatrice ^{[3
]}

Gulino, Alessandro ^{[3
]}

Gagliardo, Cesare ^{[4
]}

Florena, Ada Maria ^{[5
]}

Bilello, Giuseppa ^{[6
]}

Moschella, Francesco ^{[1
]}

Cordova, Adriana ^{[1
,2
]}

Barone, Angelo A. Leto ^{[7
]}

Toia, Francesca ^{[1
,2
]}

机构：

[1] Univ Palermo, BIOPLAST Lab BIOl & Regenerat Med PLAST Surg, Plast & Reconstruct Surg Sect, Dept Surg Oncol & Oral Sci, Palermo, Italy

[2] Azienda Osped Univ Policlin Paolo Giaccone, Plast & Reconstruct Unit, Dept Oncol, Palermo, Italy

[3] Univ Palermo, Tumor Immunol Unit, Dept Hlth Sci, Palermo, Italy

[4] Univ Palermo, Radiol Sci Sect, Dept Biopathol & Med Biotechnol, Palermo, Italy

[5] Univ Palermo, Anat Pathol Unit, Dept Hlth Sci, Dept Sci Promot Hlth & Mother & Child Care, Palermo, Italy

[6] Univ Palermo, Oral Med Unit, Dept Surg Oncol & Oral Sci, Palermo, Italy

[7] Johns Hopkins Sch Med, Dept Plast & Reconstruct Surg, 601 N Caroline St,JHOC 8152C, Baltimore, MD 21287 USA

来源：

ANNALS OF PLASTIC SURGERY | 2021年 / 86卷 / 06期

关键词：

spheroids of adipose-derived stem cells; tissue engineering; calvarial defect; bone regeneration; DERMAL REGENERATION TEMPLATE; THROMBOSPONDIN-2; SCAFFOLDS; DEFECTS; RECONSTRUCTION; SURGERY;

D O I：

10.1097/SAP.0000000000002579

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Calvarial defects can result from several causes. Tissue engineering hold the potential to restore native form and protective function. We have recently shown that stemness and differentiation ability of spheroids from adipose-derived stem cells (S-ASCs) promotes osteoblasts growth within Integra in a small vertebral lesion. In our study, we aimed to test osteogenic potential of S-ASCs in aiding regeneration of a calvarial defect. Groups containing Integra showed increased bone regeneration at the calvarial defect-Integra interface compared with the control group. In particular, S-ASC-derived osteoblasts group showed a superior calvarial remodeling than undifferentiated S-ASCs group. Clusters of ossification were observed in these both groups with enhanced microvasculature density and fibrosis. In conclusion, seeding of S-ASCs in dermal regeneration templates enhanced bone healing in a rabbit calvarial defect model. These findings could prompt the elective use of S-ASCs with enhanced multilineage differentiation potential for tissue engineering purposes.

引用

页码：714 / 720

页数：7

共 32 条

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