Bryonolic Acid: A Large-Scale Isolation and Evaluation of Heme Oxygenase 1 Expression in Activated Macrophages

被引：11

作者：

Barker, Emily C. ^{[1
]}

Gatbonton-Schwager, Tonibelle N. ^{[2
]}

Han, Yong ^{[1
]}

Clay, Jennifer E. ^{[1
]}

Letterio, John J. ^{[2
,3
]}

Tochtrop, Gregory P. ^{[1
,2
]}

机构：

[1] Case Western Reserve Univ, Dept Chem, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Dept Pharmacol, Cleveland, OH 44106 USA

[3] Case Western Reserve Univ, Dept Pediat, Cleveland, OH 44106 USA

来源：

JOURNAL OF NATURAL PRODUCTS | 2010年 / 73卷 / 06期

关键词：

CYTOTOXIC ACTIVITY; SEVERE MALARIA; CELL-CULTURES; VAR JAPONICA; HAIRY ROOTS; TRITERPENOIDS; DERIVATIVES; PATHOGENESIS; PROTECTION; SUPPRESS;

D O I：

10.1021/np1000076

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Bryonolic acid (BA) is a triterpenoid found in the Cucurbitaceae family of plants. Our interests in the immunomodulatory effects of this class of natural products led us to discover that BA induces a marked increase in the expression of it phase 2 response enzyme, heme oxygenase 1 (HO-1), in a dose-dependent manner. This phenotype has translational implications in malarial disease progression, and consequently we developed a large-scale isolation method for BA that will enable future in vitro and in vivo analyses. We have determined ideal growth conditions and time scale for maximizing BA content in the roots of Cucurbita pupa and analyzed BA production by HPLC. Large-scale extraction yielded 1.34% BA based on dry weight, allowing for the isolation of BA on a multigram scale.

引用

页码：1064 / 1068

页数：5

共 29 条

[1] Anti-tumor promoting effects of multiflorane-type triterpenoids and cytotoxic activity of karounidiol against human cancer cell lines [J].

Akihisa, T ;

Tokuda, H ;

Ichiishi, E ;

Mukainaka, T ;

Toriumi, M ;

Ukiya, M ;

Yasukawa, K ;

Nishino, H .

CANCER LETTERS, 2001, 173 (01) :9-14

[2] Arbuscular mycorrhizal fungus-promoted accumulation of two new triterpenoids in cucumber roots [J].

Akiyama, K ;

Hayashi, H .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2002, 66 (04) :762-769

[3]

BIGLINO G, 1969, Ricerca Scientifica, V39, P207

[4]

Biglino G., 1959, Ann. Chim, V49, P782

[5] FORMATION OF BRYONOLIC ACID IN CUCURBITACEOUS PLANTS AND THEIR CELL-CULTURES [J].

CHO, HJ ;

TANAKA, S ;

FUKUI, H ;

TABATA, M .

PHYTOCHEMISTRY, 1992, 31 (11) :3893-3896

[6] Heme oxygenase-1 as a therapeutic target in neurodegenerative diseases and brain infections [J].

Cuadrado, Antonio ;

Rojo, Ana I. .

CURRENT PHARMACEUTICAL DESIGN, 2008, 14 (05) :429-442

[7]

Dayal Rameshwar, 2001, Journal of Medicinal and Aromatic Plant Sciences, V23, P660

[8] Extremely potent triterpenoid inducers of the phase 2 response: Correlations of protection against oxidant and inflammatory stress [J].

Dinkova-Kostova, AT ;

Liby, KT ;

Stephenson, KK ;

Holtzclaw, WD ;

Gao, XQ ;

Suh, N ;

Williarrli, C ;

Risingsong, R ;

Honda, T ;

Gribble, GW ;

Sporn, MB ;

Talalay, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (12) :4584-4589

[9] Free radicals in the physiological control of cell function [J].

Dröge, W .

PHYSIOLOGICAL REVIEWS, 2002, 82 (01) :47-95

[10] A central role for free heme in the pathogenesis of severe malaria: the missing link? [J].

Ferreira, Ana ;

Balla, Jozsef ;

Jeney, Viktoria ;

Balla, Gyoergy ;

Soares, Miguel P. .

JOURNAL OF MOLECULAR MEDICINE-JMM, 2008, 86 (10) :1097-1111

← 1 2 3 →