Agrobacterium-Mediated High-Efficiency Genetic Transformation and Genome Editing of Chaling Common Wild Rice (Oryza rufipogon Griff.) Using Scutellum Tissue of Embryos in Mature Seeds

被引：5

作者：

Xiang, Zhipan ^{[1
]}

Chen, Yi ^{[1
]}

Chen, Yan ^{[1
]}

Zhang, Lin ^{[1
]}

Liu, Min ^{[1
]}

Mao, Dandan ^{[1
]}

Chen, Liangbi ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Life Sci, Hunan Prov Key Lab Crop Sterile Germplasm Resourc, Changsha, Peoples R China

来源：

FRONTIERS IN PLANT SCIENCE | 2022年 / 13卷

基金：

中国国家自然科学基金;

关键词：

Agrobacterium-mediated genetic transformation; genome editing; scutellum tissue of embryos in mature seeds; Chaling common wild rice; highly efficient transformation system; RESISTANCE LOCI; DRAFT SEQUENCE; DOMESTICATION; ADAPTATION; TOLERANCE; ORIGIN; SYSTEM; INDICA;

D O I：

10.3389/fpls.2022.849666

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Genetic transformation is an important strategy for revealing gene function, and it is used extensively in both functional genomics study and molecular breeding of rice. Demand for its application in wild Oryza species is rising for their extensive genetic diversity. However, genetic transformation of wild Oryza accessions with AA genome using calli induced from scutellum tissue of embryos in mature seeds has not been successfully established. In the present study, we used Chaling common wild rice (CLCWR) (Oryza rufipogon Griff.) with AA genome to successfully establish an Agrobacterium-mediated genetic transformation system based on scutellum tissue of embryos in mature seeds. The calli from embryos in mature seeds of CLCWR were easy to be induced and regenerated. The callus induction rate and texture were optimum under 2.5 mg/L 2,4-D. The optimal hormone combination used for regeneration was 2 mg/L ZT + 0.1 mg/L NAA. Studies on genetic transformation and genome editing showed that the transformation efficiency was 87-94%, the efficiency of single genome editing and multiplex genome editing were about 60-70% and 20-40%, respectively. Compared with Nipponbare (Nip), CLCWR had higher Hygromycin-resistant callus frequency and transformation efficiency. Taken together, our study establishes a highly efficient transformation system for common wild rice with AA genome and provides a good rice material for de novo domestication by genome editing in the future.

引用

页数：13