Characterization of neuropeptide F and its receptor from the African malaria mosquito, Anopheles gambiae

被引：52
作者：
Garczynski, SF
Crim, JW [1 ]
Brown, MR
机构：
[1] Univ Georgia, Dept Cellular Biol, Athens, GA 30602 USA
[2] Univ Georgia, Dept Entomol, Athens, GA 30602 USA
来源：
PEPTIDES | 2005年 / 26卷 / 01期
关键词：
insect; diptera; Anopheles; malaria; G protein-coupled receptor; neuropeptide F; neuropeptide Y;
D O I：
10.1016/j.peptides.2004.07.014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The genome of Anopheles gambiae contains sequences encoding a neuropeptide F (Ang-NPF) and NPF receptor (Ang-NPFR) related to the neuropeptide Y signaling family. cDNAs for each were cloned and sequenced. Ang-NPFR was stably expressed for radioligand binding analysis. Ang-NPF exhibited high affinity (IC50 similar to 3 nM) membrane binding; NPFs from Aedes aegypti (Aea-NPF) and Drosophila melanogaster (Drm-NPF) were less potent, with the rank order Ang-NPF > Aea-NPF > Drm-NPF > Drm-NPF8-36. RT-PCR analysis revealed Ang-NPF and Ang-NPFR transcripts in all life stages. Ang-NPF and Ang-NPFR may be strategically positioned for signaling in relation to nutritional status in the African malaria mosquito. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码：99 / 107
页数：9
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