A novel microfluidic flow-injection analysis device with fluorescence detection for cation sensing.: Application to potassium

被引：27

作者：

Destandau, Emilie ^{[1
]}

Lefevre, Jean-Pierre ^{[1
]}

Eddine, Assia Chouai Fakhr ^{[1
]}

Desportes, Serge ^{[1
]}

Jullien, Marie Caroline ^{[1
]}

Hierle, Rolland ^{[1
]}

Leray, Isabelle ^{[1
]}

Valeur, Bernard ^{[1
]}

Delaire, Jacques Alexis ^{[1
]}

机构：

[1] ENS Cachan, DAlembert Inst, F-94235 Cachan, France

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 387卷 / 08期

关键词：

mu TAS; fluorimetric detection; potassium sensor; flow-injection analysis; passive mixer;

D O I：

10.1007/s00216-007-1132-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microfabricated device has been developed for fluorimetric detection of potassium ions without previous separation. It is based on use of a fluorescent molecular sensor, calix-bodipy, specially designed to be sensitive to and selective for the target ion. The device is essentially made of a Y-shape microchannel moulded in PDMS fixed on a glass substrate. A passive mixer is used for mixing the reactant and the analyte. The optical detection arrangement uses two optical fibres, one for excitation by a light-emitting diode, the other for collection of the fluorescence. This system enabled the flow-injection analysis of the concentration of potassium ions in aqueous solutions with a detection limit of 0.5 mmol L-1 and without interference with sodium ions. A calibration plot was constructed using potassium standard solutions in the range 0-16 mmol L-1, and was used for the determination of the potassium content of a pharmaceutical pill.

引用

页码：2627 / 2632

页数：6

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