Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein

被引：18

作者：

da Silva, Patricia Bento ^{[1
]}

da Silva, Jaqueline Rodrigues ^{[1
]}

Rodrigues, Mosar Correa ^{[1
]}

Vieira, Julia Augusto ^{[1
]}

de Andrade, Ikaro Alves ^{[2
]}

Nagata, Tatsuya ^{[2
]}

Santos, Alexandre Silva ^{[3
]}

da Silva, Sebastiao William ^{[3
]}

Oliveira da Rocha, Marcia Cristina ^{[4
]}

Bao, Sonia Nair ^{[4
]}

Moraes-Vieira, Pedro M. ^{[5
]}

Proenca-Modena, Jose ^{[5
]}

Angelim, Monara K. C. ^{[5
]}

de Souza, Gabriela Fabiano ^{[5
]}

Muraro, Stefanie Primon ^{[5
]}

Branco de Barros, Andre Luis ^{[6
]}

de Souza Martins, Glendara Aparecida ^{[7
]}

Ribeiro-Dias, Fatima ^{[8
]}

Machado, Giovanna ^{[9
]}

Fessel, Melissa Regina ^{[10
]}

Chudzinski-Tavassi, Ana Marisa ^{[10
]}

Ronconi, Celia Machado ^{[11
]}

Goncalves, Debora ^{[12
]}

Curi, Rui ^{[10
,13
]}

Oliveira Jr, Osvaldo N. ^{[12
]}

Azevedo, Ricardo Bentes ^{[1
]}

机构：

[1] Univ Brasilia, Inst Biol Sci, Dept Genet & Morphol, Lab Nanobiotechnol, BR-70910900 Brasilia, DF, Brazil

[2] Univ Brasilia, Inst Biol Sci, Cell Biol Dept, Virol & Microscopy Lab, BR-70910900 Brasilia, DF, Brazil

[3] Univ Brasilia, Inst Phys, Opt Spect Lab, BR-70910900 Brasilia, DF, Brazil

[4] Univ Brasilia, Inst Biol Sci, Cell Biol Dept, Electron Microscopy Lab, BR-70910900 Brasilia, DF, Brazil

[5] Univ Estadual Campinas, Dept Genet Evolut Microbiol & Immunol, BR-13083970 Sao Paulo, Brazil

[6] Univ Fed Minas Gerais, Fac Pharm, Dept Clin & Toxicol Anal, BR-31270901 Belo Horizonte, MG, Brazil

[7] Fed Univ Tocantins, Programa Posgrad Ciencia & Tecnol Alimentos, BR-77001090 Palmas, Brazil

[8] Univ Fed Goias, Inst Patol Trop & Saude Publ, Lab Imunidade Nat LIN, BR-74690900 Goiania, Go, Brazil

[9] Ctr Tecnol Estrateg Nordeste, Av Prof Luiz Freire 01, BR-50740540 Recife, PE, Brazil

[10] Butantan Inst, Bioind Ctr, BR-05503900 Sao Paulo, SP, Brazil

[11] Univ Fed Fluminense, Dept Quim Inorgan, Campus Valonguinho S-N, BR-24020141 Niteroi, RJ, Brazil

[12] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP, Brazil

[13] Cruzeiro do Sul Univ, Interdisciplinary Program Hlth Sci, BR-01311925 Sao Paulo, Brazil

来源：

TALANTA | 2022年 / 243卷

基金：

巴西圣保罗研究基金会;

关键词：

SARS-CoV-2; Immunosensors; Bioconjugates; Spectroscopic techniques; Influenza virus; SURFACE;

D O I：

10.1016/j.talanta.2022.123355

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mass testing for the diagnosis of COVID-19 has been hampered in many countries owing to the high cost of genetic material detection. This study reports on a low-cost immunoassay for detecting SARS-CoV-2 within 30 min using dynamic light scattering (DLS). The immunosensor comprises 50-nm gold nanoparticles (AuNPs) functionalized with antibodies against SARS-CoV-2 spike glycoprotein, whose bioconjugation was confirmed using transmission electron microscopy (TEM), UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and surface-enhanced Raman scattering spectroscopy (SERS). The specific binding of the bioconjugates to the spike protein led to an increase in bioconjugate size, with a limit of detection (LOD) 5.29 x 10(3) TCID50/mL (Tissue Culture Infectious Dose). The immunosensor was also proven to be selective upon interaction with influenza viruses once no increase in size was observed after DLS measurement. The strategy proposed here aimed to use antibodies conjugated to AuNPs as a generic platform that can be extended to other detection principles, enabling technologies for low-cost mass testing for COVID-19.

引用

页数：7

共 43 条

[41] Surface-enhanced Raman scattering (SERS) for probing internal cellular structure and dynamics [J].