We have recently demonstrated that human alpha-atrial natriuretic peptide (alpha-hANP), an amyloidogenic peptide responsible for isolated atrial amyloidosis, binds to a dimeric form of apo A-I belonging to small high-density lipoproteins (HDL). This binding phenomenon is considered a protective mechanism since it inhibits or strongly reduces the ANP aggregation process. The observation that plasma exhibits at least four times greater amyloid inhibitory activity than HDL prompted us to determine whether small HDL are the only ANP plasma-binding factors. After incubation of whole plasma with labelled ANP, the macromolecular complexes were subjected to two-dimensional gel electrophoresis followed by autoradiography. The results presented here provide novel evidence of additional binding proteins, in addition to apo A-I dimer, able to bind ANP in vitro and to prevent its aggregation. The mass spectrometry analysis of the radioactive spots identified them as albumin, alpha-1 antitrypsin, orosomucoid and apo A-IV-TTR complex. The putative impact of these findings in the amyloidogenic/antiamyloidogenic peptides network is discussed.
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Bohrmann, B
Tjernberg, L
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机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Tjernberg, L
Kuner, P
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机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Kuner, P
Poli, S
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机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Poli, S
Levet-Trafit, B
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机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Levet-Trafit, B
Näslund, J
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机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Näslund, J
Richards, G
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机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Richards, G
Huber, W
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机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Huber, W
Döbeli, H
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机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Döbeli, H
Nordstedt, C
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F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, SwitzerlandF Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Bohrmann, B
Tjernberg, L
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h-index: 0
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Tjernberg, L
Kuner, P
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h-index: 0
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Kuner, P
Poli, S
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h-index: 0
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Poli, S
Levet-Trafit, B
论文数: 0引用数: 0
h-index: 0
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Levet-Trafit, B
Näslund, J
论文数: 0引用数: 0
h-index: 0
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Näslund, J
Richards, G
论文数: 0引用数: 0
h-index: 0
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Richards, G
Huber, W
论文数: 0引用数: 0
h-index: 0
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Huber, W
Döbeli, H
论文数: 0引用数: 0
h-index: 0
机构:F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland
Döbeli, H
Nordstedt, C
论文数: 0引用数: 0
h-index: 0
机构:
F Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, SwitzerlandF Hoffmann La Roche AG, Div Pharma, Preclin Res, CH-4070 Basel, Switzerland