Alpha actin isoforms expression in human and rat adult cardiac conduction system

被引：13

作者：

Orlandi, Augusto ^{[1
]}

Hao, Hiroyuki

Ferlosio, Amedeo ^{[1
]}

Clement, Sophie ^{[2
]}

Hirota, Seiichi

Spagnoli, Luigi Giusto ^{[1
]}

Gabbiani, Giulio ^{[2
]}

Chaponnier, Christine ^{[2
]}

机构：

[1] Univ Roma Tor Vergata, PTV, Dept Biopathol & Image Diagnost, Inst Pathol Anat, I-00133 Rome, Italy

[2] Univ Geneva, Fac Med, Dept Pathol & Immunol, Geneva, Switzerland

来源：

DIFFERENTIATION | 2009年 / 77卷 / 04期

基金：

瑞士国家科学基金会;

关键词：

Actin isoforms; Human myocardium; Phenotypic heterogeneity; Ageing; Conductive system; SMOOTH-MUSCLE ACTIN; EMBRYONIC HEART; SKELETAL-MUSCLE; PURKINJE-FIBERS; NEURAL CREST; IMMUNOHISTOCHEMICAL ANALYSIS; SPATIAL-DISTRIBUTION; SARCOMERIC ACTIN; MAMMALIAN HEART; CHICK-EMBRYO;

D O I：

10.1016/j.diff.2008.12.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In the adult heart, cardiac muscle comprises the working myocardium and the conduction system(CS). The latter includes the sinoatrial node (SAN), the internodal tract or bundle (IB), the atrioventricular node (AVN), the atrioventricular bundle (AVB), the bundle branches (BB) and the peripheral Purkinje fibers (PF). Most of the information concerning the phenotypic features of CS tissue derives from the characterization of avian and rodent developing hearts; data concerning the expression of actin isoforms in adult CS cardiomyocytes are scarce. Using specific antibodies, we investigated the distribution of alpha-skeletal (alpha-SKA), alpha-cardiac (alpha-CA), alpha-smooth muscle (alpha-SMA) actin isoforms and other muscle-typical proteins in the CS of human and rat hearts at different ages. SAN and IB cardiomyocytes were characterized by the presence of alpha-SMA, alpha-CA, calponin and caldesmon, whereas alpha-SKA and vimentin were absent. Double immunofluorescence demonstrated the co-localisation of alpha-SMA and alpha-CA in I-bands of SAN cardiomyocytes. AVN, AVB, BB and PF cardiomyocytes were alpha-SMA, calponin, caldesmon and vimentin negative, and alpha-CA and alpha-SKA positive. No substantial differences in actin isoform distribution were observed in human and rat hearts, except for the presence of isolated subendocardial alpha-SMA positive cardiomyocytes co-expressing alpha-CA in the ventricular septum of the rat. Aging did not influence CS cardiomyocyte actin isoform expression profile. These findings support the concept that cardiomyocytes of SAN retain the phenotype of a developing myogenic cell throughout the entire life span. (C) 2008 International Society of Differentiation. Published by Elsevier Ltd. All rights reserved.

引用

页码：360 / 368

页数：9

共 53 条

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