Development of microsatellite markers from tartary buckwheat

被引：23

作者：

Li, Yan-Qin ^{[1
]}

Shi, Tong-Lin

Zhang, Zong-Wen

机构：

[1] Shaxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China

[2] Chinese Acad Sci, IPGRI Off E Asia, Beijing 100081, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 05期

关键词：

anchored PCR; degenerate primer; Fagopyrum tataricum; microsatellite; plants diversity;

D O I：

10.1007/s10529-006-9293-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A genomic library enriched with (gT)(n) repeats from tartary buckwheat (Fagopyrum tataricum) was constructed using 5'-anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones from the library showed that they all contained microsatellites (totally 10 loci), and each was unique. An additional locus-specific primer was designed according to flanking sequence. Two of the microsatellite loci of 10 tartary buckwheat varieties were amplified using an anchored primer and a locus-specific primer, which revealed a clear polymorphic pattern. The data confirmed that the degenerate primer was reliably anchoring at the 5'-end of the microsatellite, and the primers developed based on this technology could be used for diversity analysis of tartary buckwheat.

引用

页码：823 / 827

页数：5

共 8 条

[1]

ALEKSEEVA ES, 1986, P 9 INT S BUCKWH PUL

[2]

[Anonymous], 1981, Genetika

[3] Single locus microsatellites isolated using 5' anchored PCR [J].