Sponge (2′,5′)oligoadenylate synthetase activity in the whole sponge organism and in a primary cell culture

被引:8
作者
Kelve, M [1 ]
Kuusksalu, A [1 ]
Lopp, A [1 ]
Reintamm, T [1 ]
机构
[1] NICPB, Genet Mol Lab, EE-12618 Tallinn, Estonia
关键词
2-5A synthetase; sponge; cell culture;
D O I
10.1016/S0168-1656(02)00254-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A high (2',5')oligoadenylate (2-5A) synthetase activity was found in the marine sponge Geodia cydonium. Here we demonstrate that the 2-5A synthetase activity is present also in other sponge species although the level of the 2-5A synthetase activity varies in several magnitudes in different sponges. The 2-5A synthesizing activity was maintained in the primary culture produced from a sponge. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:177 / 180
页数:4
相关论文
共 9 条
[1]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[2]   Development in a primary cell culture of the marine sponge Ircinia muscarum and analysis of the polar compounds [J].
De Rosa, S ;
De Caro, S ;
Tommonaro, G ;
Slantchev, K ;
Stefanov, K ;
Popov, S .
MARINE BIOTECHNOLOGY, 2001, 3 (03) :281-286
[3]  
GREBENJUK VA, 2002, EUR J BIOCHEM, V269, P1
[4]   Gene structure and function of the 2′-5′-oligoadenylate synthetase family [J].
Justesen, J ;
Hartmann, R ;
Kjeldgaard, NO .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2000, 57 (11) :1593-1612
[6]  
KUUSKSALU A, 1995, EUR J BIOCHEM, V232, P351, DOI 10.1111/j.1432-1033.1995.tb20818.x
[7]   Identification of the reaction products of (2′-5′)oligoadenylate synthetase in the marine sponge [J].
Kuusksalu, A ;
Subbi, J ;
Pehk, T ;
Reintamm, T ;
Müller, WEG ;
Kelve, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 257 (02) :420-426
[8]   Modulation of 2'-5'oligoadenylate synthetase by environmental stress in the marine sponge Geodia cydonium [J].
Schroder, HC ;
Wiens, M ;
Kuusksalu, A ;
Kelve, M ;
Muller, WEG .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 1997, 16 (07) :1403-1409
[9]   EXPRESSION CLONING OF 2-5A-DEPENDENT RNASE - A UNIQUELY REGULATED MEDIATOR OF INTERFERON ACTION [J].
ZHOU, AM ;
HASSEL, BA ;
SILVERMAN, RH .
CELL, 1993, 72 (05) :753-765