Sputum Detection of Predisposing Genetic Mutations in Women with Pulmonary Nontuberculous Mycobacterial Disease

被引:5
|
作者
Philley, Julie V. [1 ]
Hertweck, Kate L. [2 ]
Kannan, Anbarasu [3 ]
Brown-Elliott, Barbara A. [4 ]
Wallace, Richard J., Jr. [4 ]
Kurdowska, Anna [3 ]
Ndetan, Harrison [5 ]
Singh, Karan P. [5 ]
Miller, Edmund J. [6 ]
Griffith, David E. [1 ]
Dasgupta, Santanu [3 ]
机构
[1] Univ Texas Hlth Sci Ctr Tyler, Dept Med, Tyler, TX USA
[2] Univ Texas Tyler, Dept Biol, Tyler, TX 75799 USA
[3] Univ Texas Hlth Sci Ctr Tyler, Dept Cellular & Mol Biol, Tyler, TX 75708 USA
[4] Univ Texas Hlth Sci Ctr Tyler, Dept Microbiol, Dept Mycobacteria Nocardia Res Lab, Tyler, TX USA
[5] Univ Texas Hlth Sci Ctr Tyler, Dept Epidemiol & Biostat, Tyler, TX USA
[6] Feinstein Inst Med Res, Dept Ctr Heart & Lung Res, Manhasset, NY USA
来源
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词
BREAST-CANCER; GERMLINE MUTATIONS; AVIUM COMPLEX; RECEPTOR EXPRESSION; LUNG; CCL20; RESISTANCE; MIGRATION; DIAGNOSIS; THERAPY;
D O I
10.1038/s41598-018-29471-x
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Nontuberculous mycobacterial lung disease (NTM), including Mycobacterium avium complex (MAC), is a growing health problem in North America and worldwide. Little is known about the molecular alterations occurring in the tissue microenvironment during NTM pathogenesis. Utilizing next generation sequencing, we sequenced sputum and matched lymphocyte DNA in 15 MAC patients for a panel of 19 genes known to harbor cancer susceptibility associated mutations. Thirteen of 15 NTM subjects had a diagnosis of breast cancer (BCa) before or after NTM infection. Thirty three percent (4/12) of these NTM-BCa cases exhibited at least 3 somatic mutations in sputa compared to matched lymphocytes. Twenty four somatic mutations were detected with at least one mutation in ATM, ERBB2, BARD1, BRCA1, BRCA2, AR, TP53, PALB2, CASP8, BRIP1, NBN and TGFB1 genes. All four NTM-BCa patients harboring somatic mutations also exhibited 15 germ line BRCA1 and BRCA2 mutations. The two NTM subjects without BCa exhibited twenty somatic mutations spanning BRCA1, BRCA1, BARD1, BRIP1, CHEK2, ERBB2, TP53, ATM, PALB2, TGFB1 and 3 germ line mutations in BRCA1 and BRCA2 genes. A single copy loss of STK11 and AR gene was noted in NTM-BCa subjects. Periodic screening of sputa may aid to develop risk assessment biomarkers for neoplastic diseases in NTM patients.
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页数:10
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