Assessment of bacterial diversity associated with crude oil-contaminated soil samples from Assam
被引:11
作者:
Baruah, R.
论文数: 0引用数: 0
h-index: 0
机构:
CSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
Acad Sci & Innovat Res, New Delhi, IndiaCSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
Baruah, R.
[1
,2
]
Mishra, S. K.
论文数: 0引用数: 0
h-index: 0
机构:
Acad Sci & Innovat Res, New Delhi, India
CSIR Natl Bot Res Inst, Div Plant Microbe Interact, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, IndiaCSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
Mishra, S. K.
[2
,3
]
Kalita, D. J.
论文数: 0引用数: 0
h-index: 0
机构:
OIL, Dept Chem, Duliajan, IndiaCSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
Kalita, D. J.
[4
]
Silla, Y.
论文数: 0引用数: 0
h-index: 0
机构:
CSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, IndiaCSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
Silla, Y.
[1
]
Chauhan, P. S.
论文数: 0引用数: 0
h-index: 0
机构:
CSIR Natl Bot Res Inst, Div Plant Microbe Interact, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, IndiaCSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
Chauhan, P. S.
[3
]
Singh, A. K.
论文数: 0引用数: 0
h-index: 0
机构:
CSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, IndiaCSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
Singh, A. K.
[1
]
Boruah, H. P. Deka
论文数: 0引用数: 0
h-index: 0
机构:
CSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, IndiaCSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
Boruah, H. P. Deka
[1
]
机构:
[1] CSIR North East Inst Sci & Technol, Div Biotechnol, Jorhat 785006, Assam, India
[2] Acad Sci & Innovat Res, New Delhi, India
[3] CSIR Natl Bot Res Inst, Div Plant Microbe Interact, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, India
In this study, we are reporting bacterial diversity in crude oil-contaminated soil of Assam, India. Integration of physiological community profiling, culture-dependent and culture-independent (metagenome) approaches, was employed to obtain a complete picture of the total bacterial diversity. Samples collected from 10 sites contaminated with crude oil ranging from 0.22 to 89.36% were analysed, and altogether 160 culturable bacteria were isolated (117 Gram-positive and 43 Gram-negative bacteria). Molecular identification showed the predominance of genera Lysinibacillus, Alcaligenes, Bacillus, Clostridium, Enterobacter and Pseudomonas. Conversely, denaturing gradient gel electrophoresis (DGGE) profiles of 16S rDNA phylotypes showed the predominance of Sphingomonas, Ralstonia, Sphingobium, Massilia, Acinetobacter and Pseudomonas. Both culture-dependent and culture-independent approaches resulted in 11 genera of which Bacillus and Pseudomonas were the key inhabitants creating most favourable bacterial milieu in the presence of crude oil contaminants. Metabolic fingerprints data depicted in PCA plot demonstrated that sites CTF-D-1 and Core-10 were most diverged. It was further confirmed that variations of bacterial species dominance in different sites were due to origin of hydrocarbon contamination. We here claim that the present findings is a first-hand report on combined physiological community profiling, culture-based and culture-independent approaches in assessing total bacterial diversity in crude oil-contaminated soil of Assam.
机构:
Univ Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Univ Newcastle, Cooperat Res Ctr Environm Risk Assessment & Remed, Newcastle, NSW 2300, AustraliaUniv Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Abbasian, Firouz
;
Lockington, Robin
论文数: 0引用数: 0
h-index: 0
机构:
Univ Newcastle, Cooperat Res Ctr Environm Risk Assessment & Remed, Newcastle, NSW 2300, Australia
Univ S Australia, Fututre Ind Inst, Adelaide, SA 5001, AustraliaUniv Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Lockington, Robin
;
Megharaj, Mallavarapu
论文数: 0引用数: 0
h-index: 0
机构:
Univ Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Univ Newcastle, Cooperat Res Ctr Environm Risk Assessment & Remed, Newcastle, NSW 2300, AustraliaUniv Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Megharaj, Mallavarapu
;
Naidu, Ravi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Univ Newcastle, Cooperat Res Ctr Environm Risk Assessment & Remed, Newcastle, NSW 2300, AustraliaUniv Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
机构:
King Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi ArabiaKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia
Albokari, Majed
;
Mashhour, Ibrahim
论文数: 0引用数: 0
h-index: 0
机构:
King Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi ArabiaKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia
Mashhour, Ibrahim
;
Alshehri, Mohammed
论文数: 0引用数: 0
h-index: 0
机构:
King Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi ArabiaKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia
Alshehri, Mohammed
;
Boothman, Chris
论文数: 0引用数: 0
h-index: 0
机构:
Univ Manchester, Sch Earth Atmospher & Environm Sci, Manchester M13 9PL, Lancs, England
Univ Manchester, Williamson Res Ctr Mol Environm Sci, Manchester M13 9PL, Lancs, EnglandKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia
Boothman, Chris
;
Al-Enezi, Mousa
论文数: 0引用数: 0
h-index: 0
机构:
Saudi Aramco, Ctr Res & Dev, Dhahran 31311, Saudi ArabiaKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia
机构:
Univ Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Univ Newcastle, Cooperat Res Ctr Environm Risk Assessment & Remed, Newcastle, NSW 2300, AustraliaUniv Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Abbasian, Firouz
;
Lockington, Robin
论文数: 0引用数: 0
h-index: 0
机构:
Univ Newcastle, Cooperat Res Ctr Environm Risk Assessment & Remed, Newcastle, NSW 2300, Australia
Univ S Australia, Fututre Ind Inst, Adelaide, SA 5001, AustraliaUniv Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Lockington, Robin
;
Megharaj, Mallavarapu
论文数: 0引用数: 0
h-index: 0
机构:
Univ Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Univ Newcastle, Cooperat Res Ctr Environm Risk Assessment & Remed, Newcastle, NSW 2300, AustraliaUniv Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Megharaj, Mallavarapu
;
Naidu, Ravi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
Univ Newcastle, Cooperat Res Ctr Environm Risk Assessment & Remed, Newcastle, NSW 2300, AustraliaUniv Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
机构:
King Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi ArabiaKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia
Albokari, Majed
;
Mashhour, Ibrahim
论文数: 0引用数: 0
h-index: 0
机构:
King Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi ArabiaKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia
Mashhour, Ibrahim
;
Alshehri, Mohammed
论文数: 0引用数: 0
h-index: 0
机构:
King Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi ArabiaKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia
Alshehri, Mohammed
;
Boothman, Chris
论文数: 0引用数: 0
h-index: 0
机构:
Univ Manchester, Sch Earth Atmospher & Environm Sci, Manchester M13 9PL, Lancs, England
Univ Manchester, Williamson Res Ctr Mol Environm Sci, Manchester M13 9PL, Lancs, EnglandKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia
Boothman, Chris
;
Al-Enezi, Mousa
论文数: 0引用数: 0
h-index: 0
机构:
Saudi Aramco, Ctr Res & Dev, Dhahran 31311, Saudi ArabiaKing Abdulaziz City Sci & Technol, Atom Energy Res Inst, Riyadh 11442, Saudi Arabia