Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway

被引：96

作者：

Richard, P ^{[1
]}

Verho, R ^{[1
]}

Putkonen, M ^{[1
]}

Londesborough, J ^{[1
]}

Penttilä, M ^{[1
]}

机构：

[1] VTT Biotechnol, Espoo 02044, Finland

来源：

FEMS YEAST RESEARCH | 2003年 / 3卷 / 02期

基金：

芬兰科学院;

关键词：

L-arabinose; pentose fermentation; L-arabinitol; 4-dehydrogenase; L-xylulose reductase; Saccharomyces cerevisiae;

D O I：

10.1016/S1567-1356(02)00184-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The fungal pathway for L-arabinose catabolism converts c-arabinose to D-xylulose 5-phosphate in five steps. The intermediates are, in this order: L-arabinitol, L-xylulose, xylitol and D-xylulose. Only some of the genes for the corresponding enzymes were known. We have recently identified the two missing genes for L-arabinitol 4-dehydrogenase and L-xylulose reductase and shown that overexpression of all the genes of the pathway in Saccharomyces cerevisiae enables growth on L-arabinose. Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：185 / 189

页数：5

共 28 条

[1] CLONING AND EXPRESSION IN SACCHAROMYCES-CEREVISIAE OF THE NAD(P)H-DEPENDENT XYLOSE REDUCTASE-ENCODING GENE (XYL1) FROM THE XYLOSE-ASSIMILATING YEAST PICHIA-STIPITIS [J].