Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

被引：46

作者：

Caschera, Filippo ^{[1
]}

Lee, Jin Woo ^{[2
]}

Ho, Kenneth K. Y. ^{[2
]}

Liu, Allen P. ^{[2
]}

Jewett, Michael C. ^{[1
]}

机构：

[1] Northwestern Univ, Dept Chem & Biochem Engn, Chem Life Proc Inst, Simpson Querrey Inst BioNanotechnol, Evanston, IL 60208 USA

[2] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 31期

关键词：

ARTIFICIAL CELLS; FREE EXPRESSION; PERMEABILITY; INTEGRATION; VESICLES; BIOLOGY;

D O I：

10.1039/c6cc00223d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.

引用

页码：5467 / 5469

页数：3

共 27 条

[1] Ultrathin Shell Double Emulsion Templated Giant Unilamellar Lipid Vesicles with Controlled Microdomain Formation
Arriaga, Laura R.
Datta, Sujit S.
Kim, Shin-Hyun
Amstad, Esther
Kodger, Thomas E.
Monroy, Francisco
Weitz, David A.
[J]. SMALL, 2014, 10 (05) : 950 - 956
[2] Integration of biological parts toward the synthesis of a minimal cell
Caschera, Filippo
Noireaux, Vincent
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2014, 22 : 85 - 91
[3] Programmed Vesicle Fusion Triggers Gene Expression
Caschera, Filippo
Sunami, Takeshi
Matsuura, Tomoaki
Suzuki, Hiroaki
Hanczyc, Martin M.
Yomo, Tetsuya
[J]. LANGMUIR, 2011, 27 (21) : 13082 - 13090
[4] Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
Coyne, Christopher W.
Patel, Karan
Heureaux, Johanna
Stachowiak, Jeanne
Fletcher, Daniel A.
Liu, Allen P.
[J]. Jove-Journal of Visualized Experiments, 2014, (84):
[5] Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways
Elani, Yuval
Law, Robert V.
Ces, Oscar
[J]. NATURE COMMUNICATIONS, 2014, 5
[6] Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction
Fritz, Brian R.
Jamil, Osman K.
Jewett, Michael C.
[J]. NUCLEIC ACIDS RESEARCH, 2015, 43 (09) : 4774 - 4784
[7] The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction
Fritz, Brian R.
Jewett, Michael C.
[J]. NUCLEIC ACIDS RESEARCH, 2014, 42 (10) : 6774 - 6785
[8] Engineering artificial cells by combining HeLa-based cell-free expression and ultrathin double emulsion template
Ho, Kenneth K. Y.
Murray, Victoria L.
Liu, Allen P.
[J]. BUILDING A CELL FROM ITS COMPONENT PARTS, 2015, 128 : 303 - 318
[9] Improving Cell-Free Protein Synthesis through Genome Engineering of Escherichia coli Lacking Release Factor 1
Hong, Seok Hoon
Kwon, Yong-Chan
Martin, Rey W.
Soye, Benjamin J. Des
de Paz, Alexandra M.
Swonger, Kirsten N.
Ntai, Ioanna
Kelleher, Neil L.
Jewett, Michael C.
[J]. CHEMBIOCHEM, 2015, 16 (05) : 844 - 853
[10] In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation
Jewett, Michael C.
Fritz, Brian R.
Timmerman, Laura E.
Church, George M.
[J]. MOLECULAR SYSTEMS BIOLOGY, 2013, 9

← 1 2 3 →