A One Year Study on the Concentrations of Norovirus and Enteric Adenoviruses in Wastewater and A Surface Drinking Water Source in Norway

被引：62

作者：

Grondahl-Rosado, Ricardo C. ^{[1
]}

Yarovitsyna, Ekaterina ^{[1
]}

Trettenes, Elin ^{[2
]}

Myrmel, Mette ^{[1
]}

Robertson, Lucy J. ^{[1
]}

机构：

[1] Norwegian Univ Life Sci, Fac Vet Sci & Biomed, Dept Food Safety & Infect Biol, N-0033 Oslo, Norway

[2] Natl Vet Inst, Virol Sect, Oslo, Norway

来源：

FOOD AND ENVIRONMENTAL VIROLOGY | 2014年 / 6卷 / 04期

关键词：

Norovirus; Human adenovirus; Drinking water; Wastewater; Quantitative PCR; Public health; REAL-TIME PCR; REVERSE-TRANSCRIPTION-PCR; UNITED-STATES; GENOGROUP-I; VIRUSES; OUTBREAKS; STANDARDIZATION; RECOVERY; REMOVAL; FILTER;

D O I：

10.1007/s12560-014-9161-5

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Enteric viruses transmitted via the faecal-oral route occur in high concentrations in wastewater and may contaminate drinking water sources and cause disease. In order to quantify enteric adenovirus and norovirus genotypes I and II (GI and GII) impacting a drinking source in Norway, samples of surface water (52), wastewater inlet (64) and outlet (59) were collected between January 2011 and April 2012. Samples were concentrated in two steps, using an electropositive disc filter and polyethylene glycol precipitation, followed by nucleic acid extraction and analysis by quantitative polymerase chain reaction. Virus was detected in 47/52 (90.4 %) of surface water, 59/64 (92 %) of wastewater inlet and 55/59 (93 %) of wastewater outlet samples. Norovirus GI occurred in the highest concentrations in surface water (2.51e + 04) and adenovirus in wastewater (2.15e + 07). While adenovirus was the most frequently detected in all matrices, norovirus GI was more frequently detected in surface water and norovirus GII in wastewater. This study is the first in Norway to monitor both sewage and a drinking water source in parallel, and confirms the year-round presence of norovirus and adenovirus in a Norwegian drinking water source.

引用

页码：232 / 245

页数：14

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