Characterization of the APETALA2/Ethylene-responsive factor (AP2/ERF) transcription factor family in sunflower

被引：51
作者：
Najafi, Somayeh [1 ]
Sorkheh, Karim [1 ]
Nasernakhaei, Fatemeh [1 ]
机构：
[1] Shahid Chamran Univ Ahvaz, Fac Agr, Dept Agron & Plant Breeding, POB 61355-144, Ahvaz, Iran
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词：
GENOME-WIDE ANALYSIS; RESPONSIVE GENE-EXPRESSION; SALT STRESS; FUNCTIONAL-CHARACTERIZATION; MOLECULAR EVOLUTION; DISEASE RESISTANCE; NEGATIVE REGULATOR; BINDING PROTEINS; DREB2-LIKE GENE; SEED YIELD;
D O I：
10.1038/s41598-018-29526-z
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
One of the most prominent families of genes in plants is the AP2/ERF which play an important role in regulating plant growth and responses to various stresses. In this research, a genome-wide survey was conducted to recognize the AP2/ERF genes in sunflower (Helianthus annuus L.), and a total of 288 HaAP2/ERF was obtained. Phylogenetic analysis divided them into four sub-families, including 248 ERF, 4 RAV and 35 AP2, and one subgroup of the Soloist family. Localization of chromosome, gene structure, the conserved motif, gene ontology, interaction networks, homology modeling, the modeling of cis-regulatory elements and the analysis of events in the duplication of genes were carried out for HaAP2/ERF genes. Finally, 9AP2/ERF genes were chosen to confirm the gene expression of the selected genes in leaf and root tissues in various abiotic stress conditions by qPCR. The results confirmed that AP2/ERFs genes could effectively resist abiotic stress. Also, proline content was studied under drought, salinity, cold and heat stress. The results indicated that proline was increased under abiotic stress. This research has been done for the first time to determine the HaAP2/ERF family, which prepared valuable data for the evolutionary and practical research regarding AP2/ERF in sunflower.
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页数：16
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