Method for loading liposomes with soybean protein isolate hydrolysate influences the antioxidant efficiency of liposomal systems: Adding after liposomes formation or before lipid film hydration

被引:16
作者
Chen, Chong [1 ,3 ,4 ]
Sun-Waterhouse, Dongxiao [1 ,2 ,5 ]
Zhao, Jie [1 ]
Zhang, Yi [1 ]
Waterhouse, Geoffrey I. N. [2 ]
Lin, Lijing [3 ,4 ]
Zhao, Mouming [1 ,5 ]
Sun, Weizheng [1 ,5 ]
机构
[1] South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510641, Peoples R China
[2] Univ Auckland, Sch Chem Sci, Auckland 1010, New Zealand
[3] Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Key Lab Trop Crop Prod Proc, Minist Agr & Rural Affairs, Zhanjiang 524001, Guangdong, Peoples R China
[4] Hainan Key Lab Storage & Proc Fruits & Vegetables, Zhanjiang 524001, Guangdong, Peoples R China
[5] Overseas Expertise Intro Ctr Discipline Innovat F, Guangzhou 510641, Peoples R China
基金
中国国家自然科学基金;
关键词
Liposomal membrane; Electrostatic interaction; Effective absorption; Secondary structure; Protein stabilizer; WHEY PEPTIDES; ENCAPSULATION; EXTRACT;
D O I
10.1016/j.foodhyd.2022.107629
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
This research investigated the method for loading liposomes with soybean protein isolate hydrolysate (SPIH) to create stable and antioxidative protein hydrolysate-containing liposomes. SPIH (SPIH-AF) prepared via a twostep hydrolysis by alcalase and flavourzyme was added at different concentrations to liposomes by two methods. More effective adsorption of SPIH-AF onto liposomal membrane occurred when it was added at 0.5 mg/ mL and distributed in both the inner and outer water phases (addition before lipid film hydration), or added at lower concentrations (< 0.192 mg/mL) and distributed in the outer water phase (addition after liposomes formation). SPIH-AF concentration and distribution directly influenced its early rate of inhibition on lipid oxidation and membrane properties. SPIH-AF in the outer water phase functioned more effectively to inhibit lipid oxidation products accumulation. The interactions between SPIH-AF and liposomes altered SPIH-AF's secondary structures. Compared with Trp-liposomes interactions, Tyr-liposomes interactions were more sensitive to SPIHAF concentration and distribution. SPIH-AF distribution affected strongly the electrostatic interactions (but weakly the hydrophobic interactions). Tyr/Trp-liposomes interactions were more powerful when SPIH-AF was located in both the inner and outer water phases at higher concentrations.
引用
收藏
页数:9
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