共 24 条
Method for loading liposomes with soybean protein isolate hydrolysate influences the antioxidant efficiency of liposomal systems: Adding after liposomes formation or before lipid film hydration
被引:16
作者:
Chen, Chong
[1
,3
,4
]
Sun-Waterhouse, Dongxiao
[1
,2
,5
]
Zhao, Jie
[1
]
Zhang, Yi
[1
]
Waterhouse, Geoffrey I. N.
[2
]
Lin, Lijing
[3
,4
]
Zhao, Mouming
[1
,5
]
Sun, Weizheng
[1
,5
]
机构:
[1] South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510641, Peoples R China
[2] Univ Auckland, Sch Chem Sci, Auckland 1010, New Zealand
[3] Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Key Lab Trop Crop Prod Proc, Minist Agr & Rural Affairs, Zhanjiang 524001, Guangdong, Peoples R China
[4] Hainan Key Lab Storage & Proc Fruits & Vegetables, Zhanjiang 524001, Guangdong, Peoples R China
[5] Overseas Expertise Intro Ctr Discipline Innovat F, Guangzhou 510641, Peoples R China
基金:
中国国家自然科学基金;
关键词:
Liposomal membrane;
Electrostatic interaction;
Effective absorption;
Secondary structure;
Protein stabilizer;
WHEY PEPTIDES;
ENCAPSULATION;
EXTRACT;
D O I:
10.1016/j.foodhyd.2022.107629
中图分类号:
O69 [应用化学];
学科分类号:
081704 ;
摘要:
This research investigated the method for loading liposomes with soybean protein isolate hydrolysate (SPIH) to create stable and antioxidative protein hydrolysate-containing liposomes. SPIH (SPIH-AF) prepared via a twostep hydrolysis by alcalase and flavourzyme was added at different concentrations to liposomes by two methods. More effective adsorption of SPIH-AF onto liposomal membrane occurred when it was added at 0.5 mg/ mL and distributed in both the inner and outer water phases (addition before lipid film hydration), or added at lower concentrations (< 0.192 mg/mL) and distributed in the outer water phase (addition after liposomes formation). SPIH-AF concentration and distribution directly influenced its early rate of inhibition on lipid oxidation and membrane properties. SPIH-AF in the outer water phase functioned more effectively to inhibit lipid oxidation products accumulation. The interactions between SPIH-AF and liposomes altered SPIH-AF's secondary structures. Compared with Trp-liposomes interactions, Tyr-liposomes interactions were more sensitive to SPIHAF concentration and distribution. SPIH-AF distribution affected strongly the electrostatic interactions (but weakly the hydrophobic interactions). Tyr/Trp-liposomes interactions were more powerful when SPIH-AF was located in both the inner and outer water phases at higher concentrations.
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页数:9
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