Reversed-phase separation of achiral isomers by varying temperature and either gradient time or solvent strength

被引：34
作者：
Snyder, LR [1 ]
Dolan, JW [1 ]
机构：
[1] LC Resources Inc, Orinda, CA 94563 USA
来源：
JOURNAL OF CHROMATOGRAPHY A | 2000年 / 892卷 / 1-2期
基金：
美国国家卫生研究院;
关键词：
temperature effects; solvent strength; mobile phase composition; gradient elution; computer simulation; resolution; stereoisomers; positional isomers; steroids; polynuclear aromatic hydrocarbons; fatty acid methyl esters; benzoic acids; anilines;
D O I：
10.1016/S0021-9673(00)00050-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The separation of several isomer pairs of widely varied structure was studied as a function of changes in temperature, gradient time, mobile phase pH, column type ("monomeric" vs. "polymeric" C-18-silica) and organic solvent (methanol vs. acetonitrile). General conclusions are drawn which may prove useful in future attempts at the separation of these and other achiral isomers. (C) 2000 Elsevier Science B.V. All rights reserved.
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页码：107 / 121
页数：15
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