Film Dressings Based on Hydrogels: Simultaneous and Sustained-Release of Bioactive Compounds with Wound Healing Properties

被引:38
作者
Avila-Salas, Fabian [1 ]
Marican, Adolfo [2 ,7 ]
Pinochet, Soledad [3 ]
Carreno, Gustavo [2 ,7 ]
Valdes, Oscar [3 ]
Venegas, Bernardo [4 ]
Donoso, Wendy [4 ]
Cabrera-Barjas, Gustavo [5 ]
Vijayakumar, Sekar [6 ]
Duran-Lara, Esteban F. [7 ,8 ]
机构
[1] Univ Mayor, Ctr Nanotecnol Aplicada, Fac Ciencias, Huechuraba 8580000, Region Metropol, Chile
[2] Univ Talca, Inst Quim Recursos Nat, Talca 3460000, Maule, Chile
[3] Univ Catolica Maule, Vicerrectoria Invest & Postgrad, Talca 3460000, Maule, Chile
[4] Univ Talca, Dept Stomatol, Fac Hlth Sci, Talca 3460000, Chile
[5] Univ Concepcion, Technol Dev Unit UDT, Av Cordillera 2634,Parque Ind Coronel, Coronel 4191996, Biobio, Chile
[6] Alagappa Univ, Nanobiosci & Nanopharmacol Div, Biomat & Biotechnol Anim Hlth Lab, Dept Anim Hlth & Management, Sci Campus 6th Floor, Karaikkudi 630004, Tamil Nadu, India
[7] Univ Talca, Bio & NanoMat Lab, Drug Delivery & Controlled Release, Talca 3460000, Maule, Chile
[8] Univ Talca, Dept Microbiol, Fac Ciencias Salud, Talca 3460000, Maule, Chile
关键词
sustained release; wound healing; crosslinking; allantoin; equilibrium swelling ratio; accumulative release; thermogravimetric analysis; MECHANICAL-PROPERTIES; DRUG-DELIVERY; INJECTABLE HYDROGELS; POLY(VINYL ALCOHOL); SWELLING BEHAVIOR; PVA; SKIN; ANTIOXIDANT; COMBINATION; GROWTH;
D O I
10.3390/pharmaceutics11090447
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
This research proposes the rational modeling, synthesis and evaluation of film dressing hydrogels based on polyvinyl alcohol crosslinked with 20 different kinds of dicarboxylic acids. These formulations would allow the sustained release of simultaneous bioactive compounds including allantoin, resveratrol, dexpanthenol and caffeic acid as a multi-target therapy in wound healing. Interaction energy calculations and molecular dynamics simulation studies allowed evaluating the intermolecular affinity of the above bioactive compounds by hydrogels crosslinked with the different dicarboxylic acids. According to the computational results, the hydrogels crosslinked with succinic, aspartic, maleic and malic acids were selected as the best candidates to be synthesized and evaluated experimentally. These four crosslinked hydrogels were prepared and characterized by FTIR, mechanical properties, SEM and equilibrium swelling ratio. The sustained release of the bioactive compounds from the film dressing was investigated in vitro and in vivo. The in vitro results indicate a good release profile for all four analyzed bioactive compounds. More importantly, in vivo experiments suggest that prepared formulations could considerably accelerate the healing rate of artificial wounds in rats. The histological studies show that these formulations help to successfully reconstruct and thicken epidermis during 14 days of wound healing. Moreover, the four film dressings developed and exhibited excellent biocompatibility. In conclusion, the novel film dressings based on hydrogels rationally designed with combinatorial and sustained release therapy could have significant promise as dressing materials for skin wound healing.
引用
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页数:19
相关论文
共 58 条
[1]  
[Anonymous], 2013, J CHEM-NY
[2]  
[Anonymous], US FDA GUIDANCE IND
[3]  
[Anonymous], BIOMED MAT
[4]  
[Anonymous], MARVINSKETCH PROGR V
[5]   Profile of wound healing process induced by allantoin [J].
Araujo, Lorena Ulhoa ;
Grabe-Guimaraes, Andrea ;
Furtado Mosqueira, Vanessa Carla ;
Carneiro, Claudia Martins ;
Silva-Barcellos, Neila Marcia .
ACTA CIRURGICA BRASILEIRA, 2010, 25 (05) :460-466
[6]   Rational Development of a Novel Hydrogel as a pH-Sensitive Controlled Release System for Nifedipine [J].
Avila-Salas, Fabian ;
Rodriguez Nunez, Yeray A. ;
Marican, Adolfo ;
Castro, Ricardo I. ;
Villasenor, Jorge ;
Santos, Leonardo S. ;
Wehinger, Sergio ;
Duran-Lara, Esteban F. .
POLYMERS, 2018, 10 (07)
[7]   Study of Interaction Energies between the PAMAM Dendrimer and Nonsteroidal Anti-Inflammatory Drug Using a Distributed Computational Strategy and Experimental Analysis by ESI-MS/MS [J].
Avila-Salas, Fabian ;
Sandoval, Claudia ;
Caballero, Julio ;
Guinez-Molinos, Sergio ;
Santos, Leonardo S. ;
Cachau, Raul E. ;
Gonzalez-Nilo, Fernando D. .
JOURNAL OF PHYSICAL CHEMISTRY B, 2012, 116 (07) :2031-2039
[8]   Hydrogels for combination delivery of antineoplastic agents [J].
Bouhadir, KH ;
Alsberg, E ;
Mooney, DJ .
BIOMATERIALS, 2001, 22 (19) :2625-2633
[9]   Hydrogels for Therapeutic Delivery: Current Developments and Future Directions [J].
Buwalda, Sytze J. ;
Vermonden, Tina ;
Hennink, Wim E. .
BIOMACROMOLECULES, 2017, 18 (02) :316-330
[10]   Biomedical applications of hydrogels: A review of patents and commercial products [J].
Calo, Enrica ;
Khutoryanskiy, Vitaliy V. .
EUROPEAN POLYMER JOURNAL, 2015, 65 :252-267