Analysis of Ion Transport through a Single Channel of Gramicidin A in Bilayer Lipid Membranes

被引：8
|
作者：
Kubota, Shintaro [1 ]
Shirai, Osamu [1 ]
Kitazumi, Yuki [1 ]
Kano, Kenji [1 ]
机构：
[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan
来源：
ANALYTICAL SCIENCES | 2016年 / 32卷 / 02期
关键词：
Gramicidin A; bilayer lipid membrane; ion transport; channel; permselectivity; antiport; voltammetry; HIGH POTENTIALS; SELECTIVITY; ANIONS;
D O I：
10.2116/analsci.32.189
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Ion transport through a single channel of gramicidin A (GA) within the bilayer lipid membrane (BLM) between two aqueous phases (W1 and W2) has been analyzed based on the electroneutrality principle. The single-channel current increases in proportion to the magnitude of the applied membrane potential and is also dependent on the permeability coefficients of electrolyte ions (K+ and Cl-). By varying the ratio of the concentration of KCl in W1 to that in W2, the ratio of the diffusion coefficient of K+ in the BLM to that of Cl- in the BLM can be evaluated.
引用
收藏
页码：189 / 192
页数：4
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