The effect of some cryoprotectants on dromedary camel frozen-thawed semen

Contents The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi camels, diluted with Shotor diluent supplemented with glycerol (Sh-G), dimethyl formamide (DMF, Sh-DF), dimethyl sulfoxide (DMSO, Sh-DS) or ethylene glycol (EG, Sh-EG), all at 6% final concentration, and the samples were subjected to cryopreservation. The results revealed the superiority of Sh-DF over Sh-G and Sh-DS in terms of post-thaw motility (55.83 +/- 2.20 vs. 47.50 +/- 4.33 and 45.00 +/- 2.89%, respectively), sperm membrane (49.00 +/- 0.58, 39.33 +/- 3.33 and 42.67 +/- 1.45%, respectively) and acrosomal integrities (53.00 +/- 0.58, 57.33 +/- 0.88 and 52.33 +/- 1.45%, respectively). Sh-EG group showed the lowest post-thaw motility, plasma membrane and acrosome integrities (12.50 +/- 1.44, 22.67 +/- 1.45 and 30.67 +/- 1.45, respectively). In conclusion, the protocols of dromedary camel semen cryopreservation could be enhanced using 6% DMF as a cryoprotectant agent.