Improvement by Human Oligodendrocyte Progenitor Cells of Neurobehavioral Disorders in an Experimental Model of Neonatal Periventricular Leukomalacia

被引：30
作者：
Kim, Tae-Kyun [1 ]
Park, Dongsun [1 ]
Ban, Young-Hwan [1 ]
Cha, Yeseul [1 ]
An, Eun Suk [1 ]
Choi, Jieun [1 ]
Choi, Ehn-Kyoung [1 ]
Kim, Yun-Bae [1 ]
机构：
[1] Chungbuk Natl Univ, Coll Vet Med, 1 Chungdaero Gaesin Dong, Cheongju 28644, Chungbuk, South Korea
来源：
CELL TRANSPLANTATION | 2018年 / 27卷 / 07期
基金：
新加坡国家研究基金会;
关键词：
periventricular leukomalacia; neurobehavioral deficit; oligodendrocyte progenitor cell (OPC); myelination; NEURAL STEM-CELLS; PERINATAL WHITE-MATTER; ISCHEMIC BRAIN-INJURY; HYPOXIA-ISCHEMIA; CEREBRAL-PALSY; COGNITIVE FUNCTION; RAT MODEL; VULNERABILITY; DIFFERENTIATION; TRANSPLANTATION;
D O I：
10.1177/0963689718781330
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The effects of human oligodendrocyte progenitor (F3.olig2) cells on improving neurobehavioral deficits were investigated in an experimental model of periventricular leukomalacia (PVL). Seven-day-old male rats were subjected to hypoxia-ischemialipopolysaccharide injection (HIL), and intracerebroventricularly transplanted with F3.olig2 (4 x 10(5) cells/rat) once at postnatal day (PND) 10 or repeatedly at PND10, 17, 27, and 37. Neurobehavioral disorders were evaluated at PND14, 20, 30, and 40 via cylinder test, locomotor activity, and rotarod performance, and cognitive function was evaluated at PND41-45 through passive avoidance and Morris water-maze performances. F3.olig2 cells recovered the rate of use of the forelimb contralateral to the injured brain, improved locomotor activity, and restored rotarod performance of PVL animals; in addition, marked improvement of learning and memory function was seen. It was confirmed that transplanted F3.olig2 cells migrated to injured areas, matured to oligodendrocytes expressing myelin basic protein (MBP), and markedly attenuated the loss of host MBP in the corpus callosum. The results indicate that the transplanted F3.olig2 cells restored neurobehavioral functions by preventing axonal demyelination, and that human oligodendrocyte progenitor cells could be a candidate for cell therapy of perinatal hypoxic-ischemic and infectious brain injuries including PVL and cerebral palsy.
引用
收藏
页码：1168 / 1177
页数：10
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