Virus neutralizing antibody response in mice and dogs with a bicistronic DNA vaccine encoding rabies virus glycoprotein and canine parvovirus VP2

被引：38
|
作者：
Patial, Sonika
Chaturvedi, V. K.
Rai, A.
Saini, M.
Chandra, Rajesh
Saini, Y.
Gupta, Praveen K. [1 ]
机构：
[1] Indian Vet Res Inst, Div Vet Biotechnol, Izatnagar 243122, Uttar Pradesh, India
[2] Indian Vet Res Inst, Div Biol Prod, Izatnagar 243122, Uttar Pradesh, India
[3] Indian Vet Res Inst, Div Biochem, Izatnagar 243122, Uttar Pradesh, India
[4] Govind Ballabh Pant Univ Agr & Technol, Dept Vet Microbiol, Pantnagar 263145, Uttar Pradesh, India
来源：
VACCINE | 2007年 / 25卷 / 20期
关键词：
bicistronic; DNA vaccine; rabies; canine parvovirus; immunization; dog; mice;
D O I：
10.1016/j.vaccine.2007.02.051
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
A bicistronic DNA vaccine against rabies and parvovirus infection of dogs was developed by subcloning rabies glycoprotein and canine parvovirus (CPV) VP2 genes into a bicistronic vector. After characterizing the expression of both the proteins in vitro, the bicistronic DNA vaccine was injected in mice and induced immune response was compared with monocistronic DNA vaccines. There was no significant difference in ELISA and virus neutralizing (VN) antibody responses against rabies and CPV in mice immunized with either bicistronic or monocistronic DNA vaccine. Further, there was significantly similar protection in mice immunized with either bicistronic or monocistronic rabies DNA vaccine on rabies virus challenge. Similarly, dogs immunized with monocistronic and bicistronic DNA vaccines developed comparable VN antibodies against rabies and CPV. This study indicated that bicistronic DNA vaccine can be used in dogs to induce virus neutralizing immune responses against both rabies and CPV. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：4020 / 4028
页数：9
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