Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its effluent-receiving river

被引：534

作者：

Xu, Jian ^{[1
,2
]}

Xu, Yan ^{[1
,3
]}

Wang, Hongmei ^{[1
]}

Guo, Changsheng ^{[1
,2
]}

Qiu, Huiyun ^{[1
,2
]}

He, Yan ^{[1
]}

Zhang, Yuan ^{[1
,2
]}

Li, Xiaochen ^{[3
]}

Meng, Wei ^{[1
,2
]}

机构：

[1] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China

[2] Chinese Res Inst Environm Sci, Lab Riverine Ecol Conservat & Technol, Beijing 100012, Peoples R China

[3] Shandong Agr Univ, Water Conservancy & Civil Engn Coll, Tai An 271000, Shandong, Peoples R China

来源：

CHEMOSPHERE | 2015年 / 119卷

基金：

美国国家科学基金会;

关键词：

Antibiotic resistant genes (ARGs); Antibiotics; Sewage treatment plant (STP); Receiving river; MUNICIPAL WASTE-WATER; VETERINARY ANTIBIOTICS; TETRACYCLINE; BACTERIA; REMOVAL; SOILS; URBAN;

D O I：

10.1016/j.chemosphere.2014.02.040

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The extensive use of antibiotics has caused the contamination of both antibiotics and antibiotic resistance genes (ARGs) in the environment. In this study, the abundance and distribution of antibiotics and ARGs from a sewage treatment plant (STP) and its effluent-receiving river in Beijing China were characterized. Three classes of antibiotics including tetracycline, sulfonamide and quinolone were quantified by LC-MS/ MS. In the secondary effluent they were detected at 195, 2001 and 3866 ng L-1, respectively, which were higher than in the receiving river water. A total of 13 ARGs (6 tet genes: tetA, tetB, tetE, tetW, tetM and tetZ, 3 sulfonamide genes: sull, sul2 and sul3, and 4 quinolone genes: gryA, parC, qnrC and qnrD) were determined by quantitative PCR. For all ARGs, sulfonamide resistance genes were present at relatively high concentrations in all samples, with the highest ARG concentration above 10(-1). ARGs remained relatively stable along each sewage treatment process. The abundances of detected ARGs from the STP were also higher than its receiving river. Bivariate correlation analysis showed that relative tet gene copies (tetB/16S-rRNA and tetW/16S-rRNA) were strongly correlated with the concentrations of tetracycline residues (r(2)>0.8, p < 0.05), while no significant correlations occurred between sulfonamides and sul genes. A negative correlation between the relative abundance of quinolone resistance gene (qnrC/16S-rRNA) and the concentrations of enrofloxacin (ENR) was also determined. The difference of ARGs levels in the raw influent and secondary effluent suggested that the STP treatment process may induce to increase the abundance of resistance genes. The results showed that the sewage was an important repository of the resistance genes, which need to be effectively treated before discharge into the natural water body. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：1379 / 1385

页数：7

共 28 条

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