Chemical correlation between Gegen Qinlian dispensing granule and its four raw herbs by LC fingerprint

被引：20

作者：

Chen, L. ^{[1
,2
]}

Tang, Y. P. ^{[1
]}

Chen, M. J. ^{[3
]}

Deng, H. S. ^{[1
,2
]}

Yan, X. P. ^{[4
]}

Wu, D. K. ^{[2
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Key Lab TCM Formulae Res, Nanjing 210046, Peoples R China

[2] Nanjing Univ Chinese Med, Coll Pharm, Nanjing 210046, Peoples R China

[3] Nanjing Univ Chinese Med, Basic Med Coll, Nanjing 210046, Peoples R China

[4] Nanjing Univ Technol, Jiangsu Prov Inst Mat Med, Nanjing 210009, Peoples R China

来源：

PHYTOMEDICINE | 2010年 / 17卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Chemical correlation; Crude herbs; Gegen Qinlian dispensing granule; LC fingerprint; LIQUID-CHROMATOGRAPHY; PUERARIA-LOBATA; MEDICINE; IDENTIFICATION; FLAVONOIDS; SEPARATION; EXTRACTS; RADIX;

D O I：

10.1016/j.phymed.2009.10.012

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Gegen Qinlian dispensing granule, a favorite composite formula, is a combination of Radix Puerariae Lobatae, Radix Scutellariae, Rhizoma Coptidis and Radix et Rhizoma Glycyrrhizae Praeparata cum Melle. To develop a method to overall evaluate correlation between the formula and its four raw herbs, LC fingerprints of the formula and its raw herbs were developed and LC-DAD-MS was employed to identify the components in the formula fingerprint. According to the characteristic fragmentation behavior of known flavonoids, alkaloids and saponins isolated from the four raw herbs as well as retention time, UV and MS data of detected compounds, a total of 23 constituents in the formula fingerprint were Structurally characterized. Chemical correlation between the formula and the four crude herbs was evaluated qualitatively and quantitatively by the developed LC fingerprints. The results showed that 8 components in the formula fingerprint were addressed to Radix Puerariae Lobatae, 11 to Radix Scutellariae, 7 to Rhizoma Coptidis, 3 to Radix et Rhizoma Glycyrrhizae Praeparata cum Melle, and that the relative area ratios of the common peaks in the formula vary slightly in comparison with corresponding ratios in its crude herbs, demonstrating the chemical constituents in the formula have patterns similar to those in its crude herbs. (C) 2009 Elsevier GmbH. All rights reserved.

引用

页码：100 / 107

页数：8

共 25 条

[1]

[Anonymous], 2000, CHIN TRADIT PAT MED, V22, P671

[2] Determination of saikosaponin derivatives in Radix bupleuri and in pharmaceuticals of the Chinese multiherb remedy Xiaochaihu-tang using liquid chromatographic tandem mass spectrometry [J].

Bao, YW ;

Li, CA ;

Shen, HW ;

Nan, FJ .

ANALYTICAL CHEMISTRY, 2004, 76 (14) :4208-4216

[3] Commonly used herbal medicines in the United States: A review [J].

Bent, S ;

Ko, R .

AMERICAN JOURNAL OF MEDICINE, 2004, 116 (07) :478-485

[4] Fast determination of flavonoids in Glycyrrhizae radix by capillary zone electrophoresis [J].

Bo, T ;

Li, KA ;

Liu, HW .

ANALYTICA CHIMICA ACTA, 2002, 458 (02) :345-354

[5] Simultaneous analysis of nine active components in Gegen Qinlian preparations by high-performance liquid chromatography with diode array detection [J].

Chen, Lihong ;

Wang, Qiang ;

Liu, Jining .

JOURNAL OF SEPARATION SCIENCE, 2006, 29 (14) :2203-2210

[6]

CHEN XH, 2009, SERIAL OBSTINATE DIS

[7]

*CHIN PHARM COMM, 2005, CHIN PHARM 2005, V1

[8]

[丛景香 CONG Jingxiang], 2005, [精细化工, Fine Chemicals], V22, P912

[9]

崔淑芬, 2007, [中成药, Chinese Traditional Patent Medicine], V29, P1636

[10] Correction of retention time shifts for chromatographic fingerprints of herbal medicines [J].

Gong, F ;

Liang, YZ ;

Fung, YS ;

Chau, FT .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1029 (1-2) :173-183

← 1 2 3 →