Immunophenotypic analysis of cerebrospinal fluid cell populations with the Cell-Dyn Sapphire haematology analyser: method feasibility and preliminary observations

被引:6
作者
Adam, P. [1 ,4 ]
Sobek, O. [1 ]
Scott, C. S. [2 ]
Dolezil, D. [3 ]
Kasik, J. [4 ]
Hajdukova, L. [1 ,4 ]
Adam, D. [1 ]
机构
[1] Inst Cerebrospinal Fluid & Neuroimmunol, Prague, Czech Republic
[2] Abbot Labs, Wiesbaden, Germany
[3] Charles Univ Prague, Dept Neurol, Med Sch 3, Prague, Czech Republic
[4] Cent Mil Hosp, Dept Neurol, Prague, Czech Republic
关键词
Cerebrospinal fluid; neuroinfective disease; immunophenotyping; fluorescence; CD-Sapphire; MONOCLONAL-ANTIBODY FLUORESCENCE; CENTRAL-NERVOUS-SYSTEM; T-CELLS; MULTIPLE-SCLEROSIS; EXPRESSION; SUBSET; NEUROBORRELIOSIS; TRAFFICKING; ENUMERATION; CAPABILITY;
D O I
10.1111/j.1751-553X.2008.01110.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
P>Cerebrospinal fluid (CSF) samples (n = 50) from patients with neurological disease (bacterial infection, viral infection, neuroborreliosis and multiple sclerosis) were analysed to characterize cell populations by fluorescent immunocytometry with the CD-Sapphire haematology analyser. Reagent combinations applied to all CSF samples comprised CD3/CD19/HLA-DR and CD4/CD8, with some being further analysed using CD3/CD4, CD3/CD16 and CD3/CD25 protocols. Of the 50 samples, 11 were excluded because of high proportions of nonviable cells (n = 2) or insufficient cell numbers (n = 9). Apart from bacterial infection with granulocytosis, all diagnostic groups showed high proportions (51.4-77.0%) of CD3+ T cells. There was a modest association between T-cell and B-cell counts, but absolute B-cell numbers exceeded 5 cells/mu l in only 7/39 cases (neuroborreliosis, n = 6; bacterial meningitis, n = 1). CD3/Ia antigen (activation) co-expression was low and only exceeded 5% in 7/39 samples with no diagnostic correlation. Primary CD4+ and CD8+ T-cell subsets showed similar quantitative trends and CD4/CD8 co-analysis revealed the presence in all diagnostic groups (neuroborreliosis and multiple sclerosis in particular) of a CD4+ CD8int fraction that was predominantly CD3+ and CD16- and had a morphological profile consistent with small lymphoid cells. Supplementary CD-Sapphire cellular immunological analysis of most CSF samples is feasible using the procedure detailed in this communication.
引用
收藏
页码:22 / 32
页数:11
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