A novel methymycin analog, 12-ketomethymycin N-oxide, produced by the heterologous expression of the large pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900

被引：0
作者：
Ueoka, Reiko [1 ]
Hashimoto, Junko [2 ]
Kozone, Ikuko [2 ]
Hashimoto, Takuya [1 ]
Kudo, Kei [1 ]
Kagaya, Noritaka [1 ]
Suenaga, Hikaru [1 ]
Ikeda, Haruo [3 ]
Shin-ya, Kazuo [1 ,4 ]
机构：
[1] Natl Inst Adv Ind Sci & Technol, Tokyo, Japan
[2] Japan Biol Informat Consortium JBIC, Tokyo, Japan
[3] Kitasato Univ, Kitasato Inst Life Sci, Sagamihara, Kanagawa, Japan
[4] Univ Tokyo, Biotechnol Res Ctr, Tokyo, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2021年 / 85卷 / 04期
关键词：
actinomycete; heterologous expression; isolation; structure elucidation; 16-MEMBERED MACROLIDE ANTIBIOTICS; AVERMITILIS; PIKROMYCIN; GENOME; SYNTHASE; CLONING;
D O I：
10.1093/bbb/zbaa111
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel methymycin analog, 12-ketomethymycin N-oxide, was produced by the heterologous expression of the pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900 together with 12-ketomethymycin, which was only isolated by the biotransformation of the synthetic intermediate before. Their structures were determined by the spectroscopic data and the chemical derivatization. 12-Ketomethymycin showed a weak cytotoxicity against SKOV-3 and Jurkat cells, although its N-oxide analog did not show any activity. Both showed no antibacterial activities against Escherichia coli and Micrococcus luteus.
引用
收藏
页码：890 / 894
页数：5
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