Recombinant Human Erythropoietin Pretreatment Attenuates Heart Ischemia-Reperfusion Injury in Rats by Suppressing the Systemic Inflammatory Response

被引：37

作者：

Shen, Y. ^{[1
]}

Wang, Y. ^{[1
]}

Li, D. ^{[1
]}

Wang, C. ^{[1
]}

Xu, B. ^{[1
]}

Dong, G. ^{[1
]}

Huang, H. ^{[1
]}

Jing, H. ^{[1
]}

机构：

[1] Nanjing Univ, Dept Cardiothorac Surg, Jinling Hosp, Sch Clin Med, Nanjing 210002, Jiangsu Prov, Peoples R China

来源：

TRANSPLANTATION PROCEEDINGS | 2010年 / 42卷 / 05期

关键词：

NITRIC-OXIDE SYNTHASE; MYOCARDIAL ISCHEMIA; ARRHYTHMIAS;

D O I：

10.1016/j.transproceed.2009.11.050

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. Ischemia-reperfusion (I/R) injury may influence graft function after transplantation. Erythropoietin (EPO) attenuates I/R injury in various animal organs such as intestine, brain, and kidney. Objective. To evaluate the effects of pretreatment with recombinant human EPO (rhEPO) on I/R-induced heart injury. Materials and Methods. A rat model of I/R injury was established by ligating the left descending coronary artery for 30 minutes, followed by reperfusion for 4 hours. Fifty Sprague-Dawley rats were divided into 5 groups: sham operation; I/R; I/R+rhEPO, 100 U/kg; I/R+rhEPO, 1000 U/kg; and I/R+rhEPO, 5000 U/kg. Electrocardiograms were assessed continuously to note arrhythmia caused by reperfusion. Serum concentrations of interleukin (IL)-6 and IL-8, and tumor necrosis factor-alpha were measured at 2 and 4 hours after reperfusion. Results. The rhEPO-treated animals exhibited dosage-dependent significant reduction in the incidence of ventricular arrhythmia caused by reperfusion, and markedly decreased serum concentrations of IL-6, IL-8, and tumor necrosis factor-alpha (P < .05) compared with the I/R group (P < .05). Conclusion. The rhEPO attenuates myocardial I/R injury in rats, at least in part related to inhibition of the system inflammatory response.

引用

页码：1595 / 1597

页数：3

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