Endothelial-Specific Cre Mouse Models: Is Your Cre CREdibile?

被引：133
作者：
Payne, Sophie [1 ]
De Val, Sarah [1 ,2 ]
Neal, Alice [2 ]
机构：
[1] Univ Oxford, Ludwig Inst Canc Res Ltd, Nuffield Dept Med, Oxford, England
[2] Univ Oxford, Dept Physiol Anat & Genet, Oxford, England
来源：
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | 2018年 / 38卷 / 11期
基金：
英国生物技术与生命科学研究理事会;
关键词：
endothelial cells; gene expression; mice; phenotype; recombinases; SITE-SPECIFIC RECOMBINATION; BLOOD-VESSEL FORMATION; RECEPTOR TYROSINE KINASE; SMOOTH-MUSCLE-CELLS; TRANSGENIC MICE; PDGF-B; SPROUTING ANGIOGENESIS; VASCULAR ENDOTHELIUM; FUNCTIONAL-ANALYSIS; CORONARY-ARTERIES;
D O I：
10.1161/ATVBAHA.118.309669
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The field of vascular biology has gained enormous insight from the use of Cre and inducible Cre mouse models to temporally and spatially manipulate gene expression within the endothelium. Models are available to constitutively or inducibly modulate gene expression in all or a specified subset of endothelial cells. However, caution should be applied to both the selection of allele and the analysis of resultant phenotype: many similarly named Cre models have divergent activity patterns while ectopic or inconsistent Cre or inducible Cre expression can dramatically affect results. In an effort to disambiguate previous data and to provide a resource to aid appropriate experimental design, here we summarize what is known about Cre recombinase activity in the most widely used endothelial-specific Cre and Cre/ERT2 mouse models.
引用
收藏
页码：2550 / 2561
页数：12
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