Labeling of BSA and imaging of mouse T-lymphocyte as well as mouse spleen tissue by L-glutathione capped CdTe quantum dots

被引：11

作者：

Dong, Wei ^{[1
,2
]}

Ge, Xin ^{[2
]}

Wang, Meng ^{[1
]}

Xu, Shukun ^{[1
]}

机构：

[1] Northeastern Univ, Dept Chem, Shenyang 110004, Liaoning, Peoples R China

[2] Shenyang Med Coll, Shenyang 110034, Liaoning, Peoples R China

来源：

LUMINESCENCE | 2010年 / 25卷 / 01期

基金：

美国国家科学基金会;

关键词：

L-glutathione; CdTe QDs; immunolabeling; T-lymphocyte; spleen tissue; BIOLOGICAL DETECTION; NANOCRYSTALS; CELLS; NANOPARTICLES; PROTEINS;

D O I：

10.1002/bio.1144

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

L-glutathione capped highly fluorescent CdTe quantum dots (QDs) were prepared by an aqueous approach and used as fluorescent labels to link albumin bovine serum (BSA) and rat anti-mouse CD4, which was expressed on mouse T-lymphocyte and mouse spleen tissue. The sharp and narrow emission peaks showed that the as-prepared QDs have desirable dispersibility, uniformity and good fluorescence properties. Both CdTe-BSA and CdTe-CD4 conjugates showed an enhancement of fluorescence intensity over that of bare CdTe QDs. The experimental result of gel electrophoresis confirmed the successful conjugation of CdTe-BSA and CdTe-CD4. The fluorescent microscopic images of CdTe-CD4 labeled mouse T-lymphocyte cells and mouse spleen tissue were compared with that obtained from fluorescein isothiocyanate labeling. It was demonstrated that the CdTe QDs-based probe exhibited much better photostability and fluorescence intensity than fluorescein isothiocyanate, showing a good application potential in the immuno-labeling of cells and tissues. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：55 / 60

页数：6