High mortalities caused by Aeromonas veronii:identification, pathogenicity, and histopathologicalstudies in Oreochromis niloticus

被引：42
作者：
Abd El Latif, A. M. [1 ]
Elabd, Hiam [1 ]
Amin, Aziza [2 ]
Eldeen, A. I. Noor [3 ]
Shaheen, A. A. [1 ]
机构：
[1] Benha Univ, Fac Vet Med, Aquat Anim Dis & Management Dept, Moshtohor 13736, Toukh, Egypt
[2] Benha Univ, Fac Vet Med, Dept Pathol, Moshtohor 13736, Toukh, Egypt
[3] Natl Res Ctr, Hydrobiol Dept, Cairo, Egypt
来源：
AQUACULTURE INTERNATIONAL | 2019年 / 27卷 / 06期
关键词：
Identification; Aeromonas veronii; Antibiogram; Histopathology; Virulence; ANTIBIOTIC-RESISTANCE; GENUS AEROMONAS; HYDROPHILA; GENES; IDENTIFICATION; FISH; ALIGNMENT;
D O I：
10.1007/s10499-019-00429-8
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The current study planned to identify suspected causes of mass mortalities in Oreochromis niloticus at private fish farms in Port Said Governorate, Egypt, during the 2017 summer season. One hundred twenty diseased moribund O. niloticus fish were randomly collected from different locations. The examined fish showed lethargy, dark skin, bilateral exophthalmia with corneal opacity, and varying degrees of ulcers. Internal examination revealed generalized inflammation, congestion, hemorrhaging, and enlargement of most internal organs. Identification was accomplished through analytical profile index (API) 20E and polymerase chain reaction (PCR) and confirmed by sequencing and phylogenetic analysis. An antibiogram was constructed based on results from a disc diffusion test. Experimental infection was also performed and showed 80% mortality. Virulence genes were also assayed. Histopathological examination of experimentally challenged fish revealed prominent pathological lesions (mainly necrosis), degenerative changes, and cellular vacuolation. In conclusion, Aeromonas veronii can be considered one of the main causes of summer mass mortalities in O. niloticus in the study area.
引用
收藏
页码：1725 / 1737
页数：13
相关论文
共 45 条
  • [11] Variant Salmonella genomic island 1 antibiotic resistance gene cluster in Salmonella enterica serovar Albany
    Doublet, B
    Lailler, R
    Meunier, D
    Brisabois, A
    Boyd, D
    Mulvey, MR
    Chaslus-Dancla, E
    Cloeckaert, A
    [J]. EMERGING INFECTIOUS DISEASES, 2003, 9 (05) : 585 - 591
  • [12] Eissa I. A. M., 2015, Life Science Journal, V12, P90
  • [13] Honey bee pollen improves growth, immunity and protection of Nile tilapia (Oreochromis niloticus) against infection with Aeromonas hydrophila
    El-Asely, Amel M.
    Abbass, Amany A.
    Austin, Brian
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2014, 40 (02) : 500 - 506
  • [14] FAISAL M, 1989, BERL MUNCH TIERARZTL, V102, P87
  • [15] Relative distribution and conservation of genes encoding aminoglycoside-modifying enzymes in Salmonella enterica serotype Typhimurium phage type DT104
    Frana, TS
    Carlson, SA
    Griffith, RW
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (01) : 445 - 448
  • [16] Standard and real-time multiplex PCR methods for detection of trimethoprim resistance dfr genes in large collections of bacteria
    Grape, M.
    Motakefi, A.
    Pavuluri, S.
    Kahlmeter, G.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (11) : 1112 - 1118
  • [17] Increase in the prevalence of oxolinic acid resistant Acinetobacter spp. observed in a stream receiving the effluent from a freshwater trout farm following the treatment with oxolinic acid-medicated feed
    Guardabassi, L
    Dalsgaard, A
    Raffatellu, M
    Olsen, JE
    [J]. AQUACULTURE, 2000, 188 (3-4) : 205 - 218
  • [18] Genetic Diversity and Antimicrobial Resistance of Escherichia coli from Human and Animal Sources Uncovers Multiple Resistances from Human Sources
    Ibekwe, A. Mark
    Murinda, Shelton E.
    Graves, Alexandria K.
    [J]. PLOS ONE, 2011, 6 (06):
  • [19] Characterization and antimicrobial susceptibility of motile aeromonads isolated from freshwater ornamental fish showing signs of septicaemia
    Jagoda, S. S. S. de S.
    Wijewardana, T. G.
    Arulkanthan, A.
    Igarashi, Y.
    Tan, E.
    Kinoshita, S.
    Watabe, S.
    Asakawa, S.
    [J]. DISEASES OF AQUATIC ORGANISMS, 2014, 109 (02) : 127 - 137
  • [20] The Genus Aeromonas: Taxonomy, Pathogenicity, and Infection
    Janda, J. Michael
    Abbott, Sharon L.
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 2010, 23 (01) : 35 - 73
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