Ion-Selective Formation of a Guanine Quadruplex on DNA Origami Structures

被引:44
作者
Olejko, Lydia [1 ,4 ]
Cywinski, Piotr J. [2 ]
Bald, Ilko [1 ,3 ]
机构
[1] Univ Potsdam, Dept Chem, D-14476 Potsdam, Germany
[2] Fraunhofer IAP, NanoPolyPhoton, D-14476 Potsdam, Germany
[3] BAM Fed Inst Mat Res & Testing, D-12489 Berlin, Germany
[4] Humboldt Univ, Sch Analyt Sci Adlershof, D-10099 Berlin, Germany
关键词
DNA nanotechnology; FRET; G-quadruplexes; nanostructures; self-assembly; ATOMIC-FORCE MICROSCOPY; POTASSIUM-ION; TELOMERIC DNA; NANOSTRUCTURES; VISUALIZATION;
D O I
10.1002/anie.201409278
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
DNA origami nanostructures are a versatile tool that can be used to arrange functionalities with high local control to study molecular processes at a single-molecule level. Here, we demonstrate that DNA origami substrates can be used to suppress the formation of specific guanine (G) quadruplex structures from telomeric DNA. The folding of telomeres into G-quadruplex structures in the presence of monovalent cations (e.g. Na+ and K+) is currently used for the detection of K+ ions, however, with insufficient selectivity towards Na+. By means of FRET between two suitable dyes attached to the 3- and 5-ends of telomeric DNA we demonstrate that the formation of G-quadruplexes on DNA origami templates in the presence of sodium ions is suppressed due to steric hindrance. Hence, telomeric DNA attached to DNA origami structures represents a highly sensitive and selective detection tool for potassium ions even in the presence of high concentrations of sodium ions.
引用
收藏
页码:673 / 677
页数:5
相关论文
共 31 条
  • [1] Fluorescence Enhancement at Docking Sites of DNA-Directed Self-Assembled Nanoantennas
    Acuna, G. P.
    Moeller, F. M.
    Holzmeister, P.
    Beater, S.
    Lalkens, B.
    Tinnefeld, P.
    [J]. SCIENCE, 2012, 338 (6106) : 506 - 510
  • [2] Balasubramanian S, 2006, RSC BIOMOL SCI, pV
  • [3] Molecular Processes Studied at a Single-Molecule Level Using DNA Origami Nanostructures and Atomic Force Microscopy
    Bald, Ilko
    Keller, Adrian
    [J]. MOLECULES, 2014, 19 (09) : 13803 - 13823
  • [4] Cech TR, 2000, ANGEW CHEM INT EDIT, V39, P34, DOI 10.1002/(SICI)1521-3773(20000103)39:1<34::AID-ANIE34>3.0.CO
  • [5] 2-N
  • [6] ENERGIEWANDERUNG UND FLUORESZENZ
    FORSTER, T
    [J]. NATURWISSENSCHAFTEN, 1946, 33 (06) : 166 - 175
  • [7] *ZWISCHENMOLEKULARE ENERGIEWANDERUNG UND FLUORESZENZ
    FORSTER, T
    [J]. ANNALEN DER PHYSIK, 1948, 2 (1-2) : 55 - 75
  • [8] Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA Nanostructures
    Fu, Jinglin
    Liu, Minghui
    Liu, Yan
    Woodbury, Neal W.
    Yan, Hao
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (12) : 5516 - 5519
  • [9] Fluorescent amplifying recognition for DNA G-quadruplex folding with a cationic conjugated polymer: A platform for homogeneous potassium detection
    He, F
    Tang, YL
    Wang, S
    Li, YL
    Zhu, DB
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (35) : 12343 - 12346
  • [10] Self-assembled water-soluble nucleic acid probe tiles for label-free RNA hybridization assays
    Ke, Yonggang
    Lindsay, Stuart
    Chang, Yung
    Liu, Yan
    Yan, Hao
    [J]. SCIENCE, 2008, 319 (5860) : 180 - 183