Monitoring Microbial Community Structure and Dynamics during in situ U(VI) Bioremediation with a Field-Portable Microarray Analysis System

被引：29
作者：
Chandler, Darrell P. [1 ]
Kukhtin, Alexander [1 ]
Mokhiber, Rebecca [1 ]
Knickerbocker, Christopher [1 ]
Ogles, Dora [2 ]
Rudy, George [1 ]
Golova, Julia [1 ]
Long, Phil [3 ]
Peacock, Aaron [4 ]
机构：
[1] Akonni Biosyst Inc, Frederick, MD 21701 USA
[2] Microbial Insights Inc, Rockford, TN 37853 USA
[3] Pacific NW Natl Lab, Richland, WA 99354 USA
[4] Haley & Aldrich, Oak Ridge, TN 37830 USA
来源：
ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2010年 / 44卷 / 14期
关键词：
16S RIBOSOMAL-RNA; BACTERIUM GEOBACTER-METALLIREDUCENS; OLIGONUCLEOTIDE MICROARRAYS; URANIUM BIOREMEDIATION; CONTAMINATED AQUIFER; REDUCTION; DIVERSITY; NITRATE; SOIL; MICROORGANISMS;
D O I：
10.1021/es1006498
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The objective of this study was to develop and validate a simple, field-portable, microarray system for monitoring microbial community structure and dynamics in groundwater and subsurface environments, using samples representing site status before acetate injection, during Fe-reduction, in the transition from Fe- to SO(4)(2-)-reduction, and into the SO(4)(2-)-reduction phase. Limits of detection for the array are approximately 10(2)-10(3) cell equivalents of DNA per reaction. Sample-to-answer results for the field deployment were obtained in 4 h. Retrospective analysis of 50 samples showed the expected progression of microbial signatures from Fe- to SO(4)(2-) -reducers with changes in acetate amendment and in situ field conditions. The microarray response for Geobacter was highly correlated with qPCR for the same target gene (R(2) = 0.84 Microarray results were in concordance with quantitative PCR data, aqueous chemistry, site lithology, and the expected microbial community response, indicating that the field-portable microarray is an accurate indicator of microbial presence and response to in situ remediation of a uranium-contaminated site.
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收藏
页码：5516 / 5522
页数：7
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