Clarithromycin resistance stability in Helicobacter pylori:: influence of the MIC and type of mutation in the 23S rRNA

被引:24
作者
Alarcón, T [1 ]
Domingo, D [1 ]
Prieto, N [1 ]
López-Brea, M [1 ]
机构
[1] Univ Madrid, Hosp Princesa, Dept Microbiol, Madrid 28006, Spain
关键词
D O I
10.1093/jac/46.4.613
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Thirty clarithromycin-resistant Helicobacter pylori strains (MIC range 8-64 mg/L) were subcultured in a drug-free medium and the MIC was determined every five passages to detect in vitro stability of resistance. Three out of the 30 (10%) lost their resistance after 10, 13 or 18 subcultures (MIC decrease from 8 to 0.008, from 16 to 0.064 and from 32 to 0.016 mg/L). The effect of four macrolides at subinhibitory concentrations on the development of resistance was studied in H. pylori NCTC 11638 and TIGR 26695. A change in the MIC was observed only when NCTC11638 was exposed to 0.5 x MIC of erythromycin for 20 days.
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页码:613 / 616
页数:4
相关论文
共 10 条
  • [1] Alarcón T, 2000, J CLIN MICROBIOL, V38, P923
  • [2] Antibiotic resistance problems with Helicobacter pylori
    Alarcón, T
    Domingo, D
    López-Brea, M
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 1999, 12 (01) : 19 - 26
  • [3] Explaining the bias in the 23S rRNA gene mutations associated with clarithromycin resistance in clinical isolates of Helicobacter pylori
    Debets-Ossenkopp, YJ
    Brinkman, AB
    Kuipers, EJ
    Vandenbroucke-Grauls, CMJE
    Kusters, JG
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (10) : 2749 - 2751
  • [4] High frequency of mutations at position 2144 of the 23S rRNA gene is clarithromycin-resistant Helicobacter pylori strains isolated in Spain
    Domingo, D
    Alarcón, T
    Sanz, JC
    Sánchez, I
    López-Brea, M
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1998, 41 (05) : 573 - 574
  • [5] Helicobacter pylori
    Dunn, BE
    Cohen, H
    Blaser, MJ
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 1997, 10 (04) : 720 - +
  • [6] HULTEN K, 1987, ANTIMICROBIAL AGENTS, V41, P2550
  • [7] Resistance of Helicobacter pylori to antibiotics
    Megraud, F
    [J]. ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 1997, 11 : 43 - 53
  • [8] Antimicrobial treatment of H-pylori infection -: a pooled efficacy analysis of eradication therapies
    Unge, P
    [J]. EUROPEAN JOURNAL OF SURGERY, 1998, 164 : 16 - 26
  • [9] Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori
    Versalovic, J
    Shortridge, D
    Kibler, K
    Griffy, MV
    Beyer, J
    Flamm, RK
    Tanaka, SK
    Graham, DY
    Go, MF
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (02) : 477 - 480
  • [10] Clarithromycin resistance in Helicobacter pylori: Prevalence in untreated dyspeptic patients and stability in vitro
    Xia, HX
    Buckley, M
    Keane, CT
    OMorain, CA
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1996, 37 (03) : 473 - 481