Methanolic Extracts of Solieria robusta Inhibits Proliferation of Oral Cancer Ca9-22 Cells via Apoptosis and Oxidative Stress

被引：41

作者：

Yen, Yii-Huei ^{[1
]}

Farooqi, Ammad Ahmad ^{[2
]}

Li, Kun-Tzu ^{[3
]}

Butt, Ghazala ^{[4
]}

Tang, Jen-Yang ^{[5
,6
,7
,8
]}

Wu, Chang-Yi ^{[9
]}

Cheng, Yuan-Bin ^{[10
]}

Hou, Ming-Feng ^{[5
,11
,12
]}

Chang, Hsueh-Wei ^{[3
,5
,13
,14
]}

机构：

[1] Ten Chan Gen Hosp, Dept Dent, Chungli 32043, Taiwan

[2] Rashid Latif Med Coll, Lab Translat Oncol & Personalized Med, Lahore 54000, Pakistan

[3] Kaohsiung Med Univ, Dept Biomed Sci & Environm Biol, Kaohsiung 80708, Taiwan

[4] Govt Coll Univ, Dept Bot, Lahore 54000, Pakistan

[5] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Ctr Canc, Kaohsiung 80708, Taiwan

[6] Kaohsiung Med Univ, Dept Radiat Oncol, Coll Med, Fac Med, Kaohsiung 80708, Taiwan

[7] Kaohsiung Med Univ Hosp, Dept Radiat Oncol, Kaohsiung 80708, Taiwan

[8] Kaohsiung Municipal Tatung Hosp, Dept Radiat Oncol, Kaohsiung 80145, Taiwan

[9] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung 80424, Taiwan

[10] Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung 80708, Taiwan

[11] Kaohsiung Med Univ, Inst Clin Med, Kaohsiung 80708, Taiwan

[12] Kaohsiung Municipal Tatung Hosp, Dept Surg, Kaohsiung 80145, Taiwan

[13] Natl Sun Yat Sen Univ, Inst Med Sci & Technol, Kaohsiung 80424, Taiwan

[14] Kaohsiung Med Univ, Res Ctr Excellence Environm Med, Kaohsiung 80708, Taiwan

来源：

MOLECULES | 2014年 / 19卷 / 11期

关键词：

red alga; oral cancer; apoptosis; ROS; mitochondrial depolarization; DNA-DAMAGE; ANTIPROLIFERATIVE ACTIVITIES; GRACILARIA-TENUISTIPITATA; ANTIOXIDANTS; SEAWEED;

D O I：

10.3390/molecules191118721

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many red algae-derived natural products are known to have anticancer effects. The biological functions of the red alga Solieria robusta from the Karachi coast (Pakistan) remain unclear. Here, we prepared a methanolic extracts of S. robusta (MESR) to examine its possible anti-oral cancer effects and the corresponding mechanism of action. Cell viability of MESR-incubated oral cancer Ca9-22 cells was dose-responsively decreased (p < 0.001). According to a propidium iodide (PI)-based assay the cell cycle distribution was dramatically changed, especially for subG1 accumulation. Annexin V/PI assay of apoptosis using flow cytometry also showed that MESR-incubated Ca9-22 cells were dose-responsively increased (p < 0.001). For evaluation of oxidative stress in MESR-incubated Ca9-22 cells, we found that reactive oxygen species (ROS) were overexpressed dose-and time-responsively and mitochondrial depolarization was also increased (p < 0.001). Taken together, MESR showed inhibitory effects on oral cancer proliferation coupled with apoptosis and oxidative stress.

引用

页码：18721 / 18732

页数：12

共 39 条

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