Flight muscle-specific expression of act88F:: GFP in transgenic Culex quinquefasciatus Say (Diptera: Culicidae)

被引：16
作者：
Allen, ML
Christensen, BM
机构：
[1] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA
[2] Univ Wisconsin, Dept Anim Hlth & Biomed Sci, Madison, WI 53706 USA
来源：
PARASITOLOGY INTERNATIONAL | 2004年 / 53卷 / 04期
关键词：
Culex quinquefasciatus; mosquito; filariasis; actin; Hermes; germ-line transformation; green fluorescent protein (GFP);
D O I：
10.1016/j.parint.2004.04.002
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A strategy to engineer a strain of Culex mosquitoes refractory to filarial transmission is described. A requirement for success of the strategy is identification of a flight muscle-specific promoter that functions in the mosquito. A GFP marker gene under the control of the promoter region of the Drosophila melanogaster act88F gene was inserted into the genome of Culex quinquefasciatus. Transformation was confirmed by Mendelian genetics. Hybridization of a genomic Southern blot to a radiolabeled probe verified that the entire donor plasmid integrated into the mosquito genome. GFP expression in the transgenic mosquitoes was restricted to the flight muscles. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：307 / 314
页数：8
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