Isolation and Partial Characterization of a Novel Lipid Transfer Protein (LTP) and Antifungal Activity of Peptides from Chilli Pepper Seeds

被引：30

作者：

Cruz, Luana P. ^{[1
]}

Ribeiro, Suzanna F. F. ^{[1
]}

Carvalho, Andre O. ^{[1
]}

Vasconcelos, Ilka M. ^{[2
]}

Rodrigues, Rosana ^{[3
]}

Da Cunha, Maura ^{[1
]}

Gomes, Valdirene M. ^{[1
]}

机构：

[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, BR-28015602 Campos Dos Goytacazes, RJ, Brazil

[2] Univ Fed Ceara, Dept Bioquim, BR-60451970 Fortaleza, Ce, Brazil

[3] Univ Estadual Norte Fluminense, Ctr Ciencias & Tecnol Agropecuarias, BR-28015602 Campos Dos Goytacazes, RJ, Brazil

来源：

PROTEIN AND PEPTIDE LETTERS | 2010年 / 17卷 / 03期

关键词：

LTP; Capsicum annuum; plant antimicrobial peptides; yeast; filamentous fungi; ANTIMICROBIAL PEPTIDES; MEMBRANE PERMEABILIZATION; FUNGAL; EXPRESSION; CDNA; IDENTIFICATION; LOCALIZATION; PURIFICATION; ARABIDOPSIS; RESISTANCE;

D O I：

10.2174/092986610790780305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aims of this study were to isolate and characterize peptides present in chilli pepper seeds and evaluate their antifungal activities. An isolated peptide closer to 9 kDa showed high sequence homology to the antimicrobial peptide lipid transfer protein. The peptide fraction containing the LTP inhibited the growth of the fungi, Fusarium oxysporum, Colletotrium lindemunthianum, the yeasts, Saccharomyces cerevisiae, Pichia membranifaciens, Candida tropicalis, Candida albicans, inhibited glucose-stimulated acidification of the medium by yeast cells of S. cerevisiae and caused several morphological changes in P. membranifaciens.

引用

页码：311 / 318

页数：8

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