Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction

被引：3

作者：

Antov, Mirjana G. ^{[1
]}

Ivetic, Darjana Z. ^{[1
]}

Jugovic, Zorica D. Knezevic ^{[2
]}

机构：

[1] Univ Novi Sad, Fac Technol, Blvd Cara Lazara 1, Novi Sad 21000, Serbia

[2] Univ Belgrade, Fac Technol & Met, Karnegijeva 4, Belgrade 11000, Serbia

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2016年 / 51卷 / 04期

关键词：

bioseparation; Aqueous two-phase extraction; partitioning; recovery; lipase; MICROBIAL LIPASES; SYSTEMS; PURIFICATION; FERMENTATION; PARTITION; BACILLUS;

D O I：

10.1080/01496395.2015.1117106

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w(-1)) polyethylene glycol 4000/15% (w w(-1)) KH2PO4/70% (w w(-1)) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery.

引用

页码：622 / 628

页数：7

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