Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction

被引：3
|
作者：
Antov, Mirjana G. [1 ]
Ivetic, Darjana Z. [1 ]
Jugovic, Zorica D. Knezevic [2 ]
机构：
[1] Univ Novi Sad, Fac Technol, Blvd Cara Lazara 1, Novi Sad 21000, Serbia
[2] Univ Belgrade, Fac Technol & Met, Karnegijeva 4, Belgrade 11000, Serbia
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2016年 / 51卷 / 04期
关键词：
bioseparation; Aqueous two-phase extraction; partitioning; recovery; lipase; MICROBIAL LIPASES; SYSTEMS; PURIFICATION; FERMENTATION; PARTITION; BACILLUS;
D O I：
10.1080/01496395.2015.1117106
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w(-1)) polyethylene glycol 4000/15% (w w(-1)) KH2PO4/70% (w w(-1)) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery.
引用
收藏
页码：622 / 628
页数：7
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