In vitro nanobody discovery for integral membrane protein targets

被引：37

作者：

Doshi, Rupak ^{[1
]}

Chen, Beverly R. ^{[1
]}

Vibat, Cecile Rose T. ^{[2
]}

Huang, Norman ^{[3
]}

Lee, Chang-Wook ^{[1
]}

Chang, Geoffrey ^{[1
,4
]}

机构：

[1] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, San Diego, CA 92093 USA

[2] Vibat Consult, Del Mar, CA 92014 USA

[3] Univ Calif San Diego, Dept Bioengn, San Diego, CA 92093 USA

[4] Univ Calif San Diego, Dept Pharmacol, San Diego, CA 92093 USA

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

关键词：

MESSENGER-RNA DISPLAY; SELECTION; EVOLUTION; GENERATION; PEPTIDES;

D O I：

10.1038/srep06760

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nanobodies (Nbs) or single-domain antibodies are among the smallest and most stable binder scaffolds known. In vitro display is a powerful antibody discovery technique used worldwide. We describe the first adaptation of in vitro mRNA/cDNA display for the rapid, automatable discovery of Nbs against desired targets, and use it to discover the first ever reported nanobody against the human full-length glucose transporter, GLUT-1. We envision our streamlined method as a bench-top platform technology, in combination with various molecular evolution techniques, for expedited Nb discovery.

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