Novel Duplex Real-Time PCR Assay Detects Bordetella holmesii in Specimens from Patients with Pertussis-Like Symptoms in Ontario, Canada

被引:54
作者
Guthrie, J. L. [1 ]
Robertson, A. V. [2 ]
Tang, P. [1 ]
Jamieson, F. [1 ,3 ]
Drews, S. J. [4 ,5 ]
机构
[1] Ontario Agcy Hlth Protect & Promot, Toronto, ON M9P 3T1, Canada
[2] Appl Biosyst Inc, Foster City, CA USA
[3] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada
[4] ProvLab, Calgary, AB, Canada
[5] Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
关键词
PARAPERTUSSIS; BACTEREMIA; SEPTICEMIA; IS481;
D O I
10.1128/JCM.02417-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bordetella holmesii is a human pathogen found mainly in immunocompromised patients. A specific real-time PCR assay was developed and successfully used to identify specimens from which B. holmesii was misidentified as Bordetella pertussis and to establish the prevalence of B. holmesii in Ontario patients with pertussis-like symptoms.
引用
收藏
页码:1435 / 1437
页数:3
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